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Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa

Phaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from...

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Autores principales: Zhang, Huajun, Peng, Yun, Zhang, Su, Cai, Guanjing, Li, Yi, Yang, Xujun, Yang, Ke, Chen, Zhangran, Zhang, Jun, Wang, Hui, Zheng, Tianling, Zheng, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844626/
https://www.ncbi.nlm.nih.gov/pubmed/27199932
http://dx.doi.org/10.3389/fmicb.2016.00602
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author Zhang, Huajun
Peng, Yun
Zhang, Su
Cai, Guanjing
Li, Yi
Yang, Xujun
Yang, Ke
Chen, Zhangran
Zhang, Jun
Wang, Hui
Zheng, Tianling
Zheng, Wei
author_facet Zhang, Huajun
Peng, Yun
Zhang, Su
Cai, Guanjing
Li, Yi
Yang, Xujun
Yang, Ke
Chen, Zhangran
Zhang, Jun
Wang, Hui
Zheng, Tianling
Zheng, Wei
author_sort Zhang, Huajun
collection PubMed
description Phaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from the bacterial cells showed strong algicidal activity. After purification, the compound showed a similar structure to prodigiosin when identified with Q-Exactive Orbitrap MS and nuclear magnetic resonance spectra. The compound showed algicidal activity against P. globosa with a 50% Lethal Dose (LD(50)) of 2.24 μg/mL. The prodigiosin was stable under heat and acid environment, and it could be degraded under alkaline environment and natural light condition. The growth rates of strain KA22 was fast in 2216E medium and the content of prodigiosin in this medium was more than 70 μg/mL after 16 h incubation. The compound showed particularly strong algicidal activity against Prorocentrum donghaiense, P. Globosa, and Heterosigma akashiwo, but having little effect on three other phytoplankton species tested. The results of our research could increase our knowledge on harmful algal bloom control compound and lead to further study on the mechanisms of the lysis effect on harmful algae.
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spelling pubmed-48446262016-05-19 Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa Zhang, Huajun Peng, Yun Zhang, Su Cai, Guanjing Li, Yi Yang, Xujun Yang, Ke Chen, Zhangran Zhang, Jun Wang, Hui Zheng, Tianling Zheng, Wei Front Microbiol Microbiology Phaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from the bacterial cells showed strong algicidal activity. After purification, the compound showed a similar structure to prodigiosin when identified with Q-Exactive Orbitrap MS and nuclear magnetic resonance spectra. The compound showed algicidal activity against P. globosa with a 50% Lethal Dose (LD(50)) of 2.24 μg/mL. The prodigiosin was stable under heat and acid environment, and it could be degraded under alkaline environment and natural light condition. The growth rates of strain KA22 was fast in 2216E medium and the content of prodigiosin in this medium was more than 70 μg/mL after 16 h incubation. The compound showed particularly strong algicidal activity against Prorocentrum donghaiense, P. Globosa, and Heterosigma akashiwo, but having little effect on three other phytoplankton species tested. The results of our research could increase our knowledge on harmful algal bloom control compound and lead to further study on the mechanisms of the lysis effect on harmful algae. Frontiers Media S.A. 2016-04-26 /pmc/articles/PMC4844626/ /pubmed/27199932 http://dx.doi.org/10.3389/fmicb.2016.00602 Text en Copyright © 2016 Zhang, Peng, Zhang, Cai, Li, Yang, Yang, Chen, Zhang, Wang, Zheng and Zheng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Huajun
Peng, Yun
Zhang, Su
Cai, Guanjing
Li, Yi
Yang, Xujun
Yang, Ke
Chen, Zhangran
Zhang, Jun
Wang, Hui
Zheng, Tianling
Zheng, Wei
Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title_full Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title_fullStr Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title_full_unstemmed Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title_short Algicidal Effects of Prodigiosin on the Harmful Algae Phaeocystis globosa
title_sort algicidal effects of prodigiosin on the harmful algae phaeocystis globosa
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844626/
https://www.ncbi.nlm.nih.gov/pubmed/27199932
http://dx.doi.org/10.3389/fmicb.2016.00602
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