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Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection
We report on highly disordered array of Au coated silicon nanowires (Au/SiNWs) as surface enhanced Raman scattering (SERS) probe combined with electrochemical detection for biosensing applications. SiNWs, few microns long, were grown by plasma enhanced chemical vapor deposition on common microscope...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844982/ https://www.ncbi.nlm.nih.gov/pubmed/27112197 http://dx.doi.org/10.1038/srep25099 |
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author | Convertino, Annalisa Mussi, Valentina Maiolo, Luca |
author_facet | Convertino, Annalisa Mussi, Valentina Maiolo, Luca |
author_sort | Convertino, Annalisa |
collection | PubMed |
description | We report on highly disordered array of Au coated silicon nanowires (Au/SiNWs) as surface enhanced Raman scattering (SERS) probe combined with electrochemical detection for biosensing applications. SiNWs, few microns long, were grown by plasma enhanced chemical vapor deposition on common microscope slides and covered by Au evaporated film, 150 nm thick. The capability of the resulting composite structure to act as SERS biosensor was studied via the biotin-avidin interaction: the Raman signal obtained from this structure allowed to follow each surface modification step as well as to detect efficiently avidin molecules over a broad range of concentrations from micromolar down to the nanomolar values. The metallic coverage wrapping SiNWs was exploited also to obtain a dual detection of the same bioanalyte by electrochemical impedance spectroscopy (EIS). Indeed, the SERS signal and impedance modifications induced by the biomolecule perturbations on the metalized surface of the NWs were monitored on the very same three-electrode device with the Au/SiNWs acting as both working electrode and SERS probe. |
format | Online Article Text |
id | pubmed-4844982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48449822016-04-29 Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection Convertino, Annalisa Mussi, Valentina Maiolo, Luca Sci Rep Article We report on highly disordered array of Au coated silicon nanowires (Au/SiNWs) as surface enhanced Raman scattering (SERS) probe combined with electrochemical detection for biosensing applications. SiNWs, few microns long, were grown by plasma enhanced chemical vapor deposition on common microscope slides and covered by Au evaporated film, 150 nm thick. The capability of the resulting composite structure to act as SERS biosensor was studied via the biotin-avidin interaction: the Raman signal obtained from this structure allowed to follow each surface modification step as well as to detect efficiently avidin molecules over a broad range of concentrations from micromolar down to the nanomolar values. The metallic coverage wrapping SiNWs was exploited also to obtain a dual detection of the same bioanalyte by electrochemical impedance spectroscopy (EIS). Indeed, the SERS signal and impedance modifications induced by the biomolecule perturbations on the metalized surface of the NWs were monitored on the very same three-electrode device with the Au/SiNWs acting as both working electrode and SERS probe. Nature Publishing Group 2016-04-26 /pmc/articles/PMC4844982/ /pubmed/27112197 http://dx.doi.org/10.1038/srep25099 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Convertino, Annalisa Mussi, Valentina Maiolo, Luca Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title | Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title_full | Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title_fullStr | Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title_full_unstemmed | Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title_short | Disordered array of Au covered Silicon nanowires for SERS biosensing combined with electrochemical detection |
title_sort | disordered array of au covered silicon nanowires for sers biosensing combined with electrochemical detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844982/ https://www.ncbi.nlm.nih.gov/pubmed/27112197 http://dx.doi.org/10.1038/srep25099 |
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