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Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses
Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mamm...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845017/ https://www.ncbi.nlm.nih.gov/pubmed/27113297 http://dx.doi.org/10.1038/srep24257 |
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author | Sasaki, Michihito Gonzalez, Gabriel Wada, Yuji Setiyono, Agus Handharyani, Ekowati Rahmadani, Ibenu Taha, Siswatiana Adiani, Sri Latief, Munira Kholilullah, Zainal Abidin Subangkit, Mawar Kobayashi, Shintaro Nakamura, Ichiro Kimura, Takashi Orba, Yasuko Ito, Kimihito Sawa, Hirofumi |
author_facet | Sasaki, Michihito Gonzalez, Gabriel Wada, Yuji Setiyono, Agus Handharyani, Ekowati Rahmadani, Ibenu Taha, Siswatiana Adiani, Sri Latief, Munira Kholilullah, Zainal Abidin Subangkit, Mawar Kobayashi, Shintaro Nakamura, Ichiro Kimura, Takashi Orba, Yasuko Ito, Kimihito Sawa, Hirofumi |
author_sort | Sasaki, Michihito |
collection | PubMed |
description | Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mammals. In this study, we performed nested-PCR screening and identified bufavirus from 12 megabats of Pteropus spp. in Indonesia. Furthermore, we determined nearly the full genome sequence of a novel megabat-borne bufavirus, tentatively named megabat bufavirus 1. Phylogenetic analyses showed that megabat bufavirus 1 clustered with known protoparvoviruses, including human bufavirus but represented a distinct lineage of bufavirus. Our analyses also inferred phylogenetic relationships among animal-borne bufaviruses recently reported by other studies. Recombination analyses suggested that the most common recent ancestor of megabat bufavirus 1 might have arisen from multiple genetic recombination events. These results characterized megabat bufavirus 1 as the first protoparvovirus discovered from megabats and indicates the high genetic divergence of bufavirus. |
format | Online Article Text |
id | pubmed-4845017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48450172016-04-29 Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses Sasaki, Michihito Gonzalez, Gabriel Wada, Yuji Setiyono, Agus Handharyani, Ekowati Rahmadani, Ibenu Taha, Siswatiana Adiani, Sri Latief, Munira Kholilullah, Zainal Abidin Subangkit, Mawar Kobayashi, Shintaro Nakamura, Ichiro Kimura, Takashi Orba, Yasuko Ito, Kimihito Sawa, Hirofumi Sci Rep Article Bufavirus is a recently recognized member of the genus Protoparvovirus in the subfamily Parvovirinae. It has been reported that human bufavirus was detected predominantly in patients with diarrhoea in several countries. However, little is known about bufavirus or its close relatives in nonhuman mammals. In this study, we performed nested-PCR screening and identified bufavirus from 12 megabats of Pteropus spp. in Indonesia. Furthermore, we determined nearly the full genome sequence of a novel megabat-borne bufavirus, tentatively named megabat bufavirus 1. Phylogenetic analyses showed that megabat bufavirus 1 clustered with known protoparvoviruses, including human bufavirus but represented a distinct lineage of bufavirus. Our analyses also inferred phylogenetic relationships among animal-borne bufaviruses recently reported by other studies. Recombination analyses suggested that the most common recent ancestor of megabat bufavirus 1 might have arisen from multiple genetic recombination events. These results characterized megabat bufavirus 1 as the first protoparvovirus discovered from megabats and indicates the high genetic divergence of bufavirus. Nature Publishing Group 2016-04-26 /pmc/articles/PMC4845017/ /pubmed/27113297 http://dx.doi.org/10.1038/srep24257 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Sasaki, Michihito Gonzalez, Gabriel Wada, Yuji Setiyono, Agus Handharyani, Ekowati Rahmadani, Ibenu Taha, Siswatiana Adiani, Sri Latief, Munira Kholilullah, Zainal Abidin Subangkit, Mawar Kobayashi, Shintaro Nakamura, Ichiro Kimura, Takashi Orba, Yasuko Ito, Kimihito Sawa, Hirofumi Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title | Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title_full | Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title_fullStr | Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title_full_unstemmed | Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title_short | Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
title_sort | divergent bufavirus harboured in megabats represents a new lineage of parvoviruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845017/ https://www.ncbi.nlm.nih.gov/pubmed/27113297 http://dx.doi.org/10.1038/srep24257 |
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