Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering

Purpose: The goal of this study has been to evaluate adipose tissue derived stem cells (ADSC(s)) from infrapatellar fat pad and characterize their cell surface markers using anti-human antibodies, as adipose tissue derived stem cells (ADSC(s)) have great potential for cellular therapies to restore i...

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Autores principales: Vahedi, Parviz, Soleimanirad, Jafar, Roshangar, Leila, Shafaei, Hajar, Jarolmasjed, Seyedhosein, Nozad Charoudeh, Hojjatollah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845544/
https://www.ncbi.nlm.nih.gov/pubmed/27123425
http://dx.doi.org/10.15171/apb.2016.016
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author Vahedi, Parviz
Soleimanirad, Jafar
Roshangar, Leila
Shafaei, Hajar
Jarolmasjed, Seyedhosein
Nozad Charoudeh, Hojjatollah
author_facet Vahedi, Parviz
Soleimanirad, Jafar
Roshangar, Leila
Shafaei, Hajar
Jarolmasjed, Seyedhosein
Nozad Charoudeh, Hojjatollah
author_sort Vahedi, Parviz
collection PubMed
description Purpose: The goal of this study has been to evaluate adipose tissue derived stem cells (ADSC(s)) from infrapatellar fat pad and characterize their cell surface markers using anti-human antibodies, as adipose tissue derived stem cells (ADSC(s)) have great potential for cellular therapies to restore injured tissues. Methods: Adipose tissue was obtained from infrapatellar fat pad of sheep. Surface markers evaluated by flow cytometry. In order to evaluate cell adhesion, the Polycaprolactone (PCL) was sterilized under Ultraviolet (UV) light and about 1×10(5) cells were seeded on PCL. Then, ASCs- PCL construct were evaluated by Scanning Electron Microscopy (Mira3 Te Scan, Czech Republic). Results: We showed that adipose tissue derived stem cells (ADSC(s)) maintain their fibroblastic-like morphology during different subcultures and cell adhesion. They were positive for CD44 and CD90 markers and negative for CD31 and Cd45 markers by human antibodies. Conclusion: Our results suggest that ASCs surface markers can be characterized by anti-human antibodies in sheep. As stem cells, they can be used in tissue engineering.
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spelling pubmed-48455442016-04-27 Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering Vahedi, Parviz Soleimanirad, Jafar Roshangar, Leila Shafaei, Hajar Jarolmasjed, Seyedhosein Nozad Charoudeh, Hojjatollah Adv Pharm Bull Research Article Purpose: The goal of this study has been to evaluate adipose tissue derived stem cells (ADSC(s)) from infrapatellar fat pad and characterize their cell surface markers using anti-human antibodies, as adipose tissue derived stem cells (ADSC(s)) have great potential for cellular therapies to restore injured tissues. Methods: Adipose tissue was obtained from infrapatellar fat pad of sheep. Surface markers evaluated by flow cytometry. In order to evaluate cell adhesion, the Polycaprolactone (PCL) was sterilized under Ultraviolet (UV) light and about 1×10(5) cells were seeded on PCL. Then, ASCs- PCL construct were evaluated by Scanning Electron Microscopy (Mira3 Te Scan, Czech Republic). Results: We showed that adipose tissue derived stem cells (ADSC(s)) maintain their fibroblastic-like morphology during different subcultures and cell adhesion. They were positive for CD44 and CD90 markers and negative for CD31 and Cd45 markers by human antibodies. Conclusion: Our results suggest that ASCs surface markers can be characterized by anti-human antibodies in sheep. As stem cells, they can be used in tissue engineering. Tabriz University of Medical Sciences 2016-03 2016-03-17 /pmc/articles/PMC4845544/ /pubmed/27123425 http://dx.doi.org/10.15171/apb.2016.016 Text en ©2016 The Authors. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Research Article
Vahedi, Parviz
Soleimanirad, Jafar
Roshangar, Leila
Shafaei, Hajar
Jarolmasjed, Seyedhosein
Nozad Charoudeh, Hojjatollah
Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title_full Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title_fullStr Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title_full_unstemmed Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title_short Advantages of Sheep Infrapatellar Fat Pad Adipose Tissue Derived Stem Cells in Tissue Engineering
title_sort advantages of sheep infrapatellar fat pad adipose tissue derived stem cells in tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845544/
https://www.ncbi.nlm.nih.gov/pubmed/27123425
http://dx.doi.org/10.15171/apb.2016.016
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