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Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells

The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources...

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Autores principales: Mohammad, Maeda H, Al-shammari, Ahmed M, Al-Juboory, Ahmad Adnan, Yaseen, Nahi Y
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846075/
https://www.ncbi.nlm.nih.gov/pubmed/27143939
http://dx.doi.org/10.2147/SCCAA.S94545
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author Mohammad, Maeda H
Al-shammari, Ahmed M
Al-Juboory, Ahmad Adnan
Yaseen, Nahi Y
author_facet Mohammad, Maeda H
Al-shammari, Ahmed M
Al-Juboory, Ahmad Adnan
Yaseen, Nahi Y
author_sort Mohammad, Maeda H
collection PubMed
description The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources for the further treatment of central nervous system defects and injuries. The mouse bone marrow MSCs were used as the source of the stem cells in this study. β-Mercaptoethanol (BME) was used as the main inducer of the neurogenesis pathway to induce neural cells and to identify NSCs. Three types of neural markers were used: nestin as the immaturation stage marker, neurofilament light chain as the early neural marker, and microtubule-associated protein 2 as the maturation marker through different time intervals in the neurogenesis process starting from the MSCs, (as undifferentiated cells), NSCs, production stages, and toward neuron cells (as differentiated cells). The results of different exposure times to BME of the neural markers analysis done by immunocytochemistry and real time-polymerase chain reaction helped us to identify the exact timing for the neural stemness state. The results showed that the best exposure time that may be used for the production of NSCs was 6 hours. The best maintenance media for NSCs were also identified. Furthermore, we optimized exposure to BME with different times and concentrations, which could be an interesting way to modulate specific neuronal differentiation and obtain autologous neuronal phenotypes. This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects.
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spelling pubmed-48460752016-05-03 Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells Mohammad, Maeda H Al-shammari, Ahmed M Al-Juboory, Ahmad Adnan Yaseen, Nahi Y Stem Cells Cloning Original Research The in vitro isolation, identification, differentiation, and neurogenesis characterization of the sources of mesenchymal stem cells (MSCs) were investigated to produce two types of cells in culture: neural cells and neural stem cells (NSCs). These types of stem cells were used as successful sources for the further treatment of central nervous system defects and injuries. The mouse bone marrow MSCs were used as the source of the stem cells in this study. β-Mercaptoethanol (BME) was used as the main inducer of the neurogenesis pathway to induce neural cells and to identify NSCs. Three types of neural markers were used: nestin as the immaturation stage marker, neurofilament light chain as the early neural marker, and microtubule-associated protein 2 as the maturation marker through different time intervals in the neurogenesis process starting from the MSCs, (as undifferentiated cells), NSCs, production stages, and toward neuron cells (as differentiated cells). The results of different exposure times to BME of the neural markers analysis done by immunocytochemistry and real time-polymerase chain reaction helped us to identify the exact timing for the neural stemness state. The results showed that the best exposure time that may be used for the production of NSCs was 6 hours. The best maintenance media for NSCs were also identified. Furthermore, we optimized exposure to BME with different times and concentrations, which could be an interesting way to modulate specific neuronal differentiation and obtain autologous neuronal phenotypes. This study was able to characterize NSCs in culture under differentiation for neurogenesis in the pathway of the neural differentiation process by studying the expressed neural genes and the ability to maintain these NSCs in culture for further differentiation in thousands of functional neurons for the treatment of brain and spinal cord injuries and defects. Dove Medical Press 2016-04-18 /pmc/articles/PMC4846075/ /pubmed/27143939 http://dx.doi.org/10.2147/SCCAA.S94545 Text en © 2016 Mohammad et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Mohammad, Maeda H
Al-shammari, Ahmed M
Al-Juboory, Ahmad Adnan
Yaseen, Nahi Y
Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_full Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_fullStr Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_full_unstemmed Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_short Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
title_sort characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846075/
https://www.ncbi.nlm.nih.gov/pubmed/27143939
http://dx.doi.org/10.2147/SCCAA.S94545
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