Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [(11)C]flumazenil-PET and histology indicat...

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Autores principales: Nishino, Asuka, Tajima, Yosuke, Takuwa, Hiroyuki, Masamoto, Kazuto, Taniguchi, Junko, Wakizaka, Hidekatsu, Kokuryo, Daisuke, Urushihata, Takuya, Aoki, Ichio, Kanno, Iwao, Tomita, Yutaka, Suzuki, Norihiro, Ikoma, Yoko, Ito, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846861/
https://www.ncbi.nlm.nih.gov/pubmed/27116932
http://dx.doi.org/10.1038/srep25072
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author Nishino, Asuka
Tajima, Yosuke
Takuwa, Hiroyuki
Masamoto, Kazuto
Taniguchi, Junko
Wakizaka, Hidekatsu
Kokuryo, Daisuke
Urushihata, Takuya
Aoki, Ichio
Kanno, Iwao
Tomita, Yutaka
Suzuki, Norihiro
Ikoma, Yoko
Ito, Hiroshi
author_facet Nishino, Asuka
Tajima, Yosuke
Takuwa, Hiroyuki
Masamoto, Kazuto
Taniguchi, Junko
Wakizaka, Hidekatsu
Kokuryo, Daisuke
Urushihata, Takuya
Aoki, Ichio
Kanno, Iwao
Tomita, Yutaka
Suzuki, Norihiro
Ikoma, Yoko
Ito, Hiroshi
author_sort Nishino, Asuka
collection PubMed
description We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [(11)C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.
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spelling pubmed-48468612016-05-04 Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model Nishino, Asuka Tajima, Yosuke Takuwa, Hiroyuki Masamoto, Kazuto Taniguchi, Junko Wakizaka, Hidekatsu Kokuryo, Daisuke Urushihata, Takuya Aoki, Ichio Kanno, Iwao Tomita, Yutaka Suzuki, Norihiro Ikoma, Yoko Ito, Hiroshi Sci Rep Article We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [(11)C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO. Nature Publishing Group 2016-04-27 /pmc/articles/PMC4846861/ /pubmed/27116932 http://dx.doi.org/10.1038/srep25072 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nishino, Asuka
Tajima, Yosuke
Takuwa, Hiroyuki
Masamoto, Kazuto
Taniguchi, Junko
Wakizaka, Hidekatsu
Kokuryo, Daisuke
Urushihata, Takuya
Aoki, Ichio
Kanno, Iwao
Tomita, Yutaka
Suzuki, Norihiro
Ikoma, Yoko
Ito, Hiroshi
Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title_full Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title_fullStr Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title_full_unstemmed Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title_short Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
title_sort long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846861/
https://www.ncbi.nlm.nih.gov/pubmed/27116932
http://dx.doi.org/10.1038/srep25072
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