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Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages

Electronic cigarettes (e-cigarettes), battery-powered devices containing nicotine, glycerin, propylene glycol, flavorings, and other substances, are increasing in popularity. They pose a potential threat to the developing brain, as nicotine is a known neurotoxicant. We hypothesized that exposure to...

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Autores principales: Lauterstein, Dana E., Tijerina, Pamella B., Corbett, Kevin, Akgol Oksuz, Betul, Shen, Steven S., Gordon, Terry, Klein, Catherine B., Zelikoff, Judith T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847079/
https://www.ncbi.nlm.nih.gov/pubmed/27077873
http://dx.doi.org/10.3390/ijerph13040417
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author Lauterstein, Dana E.
Tijerina, Pamella B.
Corbett, Kevin
Akgol Oksuz, Betul
Shen, Steven S.
Gordon, Terry
Klein, Catherine B.
Zelikoff, Judith T.
author_facet Lauterstein, Dana E.
Tijerina, Pamella B.
Corbett, Kevin
Akgol Oksuz, Betul
Shen, Steven S.
Gordon, Terry
Klein, Catherine B.
Zelikoff, Judith T.
author_sort Lauterstein, Dana E.
collection PubMed
description Electronic cigarettes (e-cigarettes), battery-powered devices containing nicotine, glycerin, propylene glycol, flavorings, and other substances, are increasing in popularity. They pose a potential threat to the developing brain, as nicotine is a known neurotoxicant. We hypothesized that exposure to e-cigarettes during early life stages induce changes in central nervous system (CNS) transcriptome associated with adverse neurobiological outcomes and long-term disease states. To test the hypothesis, pregnant C57BL/6 mice were exposed daily (via whole body inhalation) throughout gestation (3 h/day; 5 days/week) to aerosols produced from e-cigarettes either with nicotine (13–16 mg/mL) or without nicotine; following birth, pups and dams were exposed together to e-cigarette aerosols throughout lactation beginning at postnatal day (PND) 4–6 and using the same exposure conditions employed during gestational exposure. Following exposure, frontal cortex recovered from ~one-month-old male and female offspring were excised and analyzed for gene expression by RNA Sequencing (RNA-Seq). Comparisons between the treatment groups revealed that e-cigarette constituents other than nicotine might be partly responsible for the observed biological effects. Transcriptome alterations in both offspring sexes and treatment groups were all significantly associated with downstream adverse neurobiological outcomes. Results from this study demonstrate that e-cigarette exposure during early life alters CNS development potentially leading to chronic neuropathology.
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spelling pubmed-48470792016-05-04 Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages Lauterstein, Dana E. Tijerina, Pamella B. Corbett, Kevin Akgol Oksuz, Betul Shen, Steven S. Gordon, Terry Klein, Catherine B. Zelikoff, Judith T. Int J Environ Res Public Health Article Electronic cigarettes (e-cigarettes), battery-powered devices containing nicotine, glycerin, propylene glycol, flavorings, and other substances, are increasing in popularity. They pose a potential threat to the developing brain, as nicotine is a known neurotoxicant. We hypothesized that exposure to e-cigarettes during early life stages induce changes in central nervous system (CNS) transcriptome associated with adverse neurobiological outcomes and long-term disease states. To test the hypothesis, pregnant C57BL/6 mice were exposed daily (via whole body inhalation) throughout gestation (3 h/day; 5 days/week) to aerosols produced from e-cigarettes either with nicotine (13–16 mg/mL) or without nicotine; following birth, pups and dams were exposed together to e-cigarette aerosols throughout lactation beginning at postnatal day (PND) 4–6 and using the same exposure conditions employed during gestational exposure. Following exposure, frontal cortex recovered from ~one-month-old male and female offspring were excised and analyzed for gene expression by RNA Sequencing (RNA-Seq). Comparisons between the treatment groups revealed that e-cigarette constituents other than nicotine might be partly responsible for the observed biological effects. Transcriptome alterations in both offspring sexes and treatment groups were all significantly associated with downstream adverse neurobiological outcomes. Results from this study demonstrate that e-cigarette exposure during early life alters CNS development potentially leading to chronic neuropathology. MDPI 2016-04-12 2016-04 /pmc/articles/PMC4847079/ /pubmed/27077873 http://dx.doi.org/10.3390/ijerph13040417 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lauterstein, Dana E.
Tijerina, Pamella B.
Corbett, Kevin
Akgol Oksuz, Betul
Shen, Steven S.
Gordon, Terry
Klein, Catherine B.
Zelikoff, Judith T.
Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title_full Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title_fullStr Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title_full_unstemmed Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title_short Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages
title_sort frontal cortex transcriptome analysis of mice exposed to electronic cigarettes during early life stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847079/
https://www.ncbi.nlm.nih.gov/pubmed/27077873
http://dx.doi.org/10.3390/ijerph13040417
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