Cargando…

Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease

Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids and amyloid pathology. Because dietary docosahexaenoic acid (DHA) has been identified as beneficial, we compared mice fed with a DHA-suppleme...

Descripción completa

Detalles Bibliográficos
Autores principales: Bascoul-Colombo, Cécile, Guschina, Irina A., Maskrey, Benjamin H., Good, Mark, O'Donnell, Valerie B., Harwood, John L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Pub. Co 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847476/
https://www.ncbi.nlm.nih.gov/pubmed/26968097
http://dx.doi.org/10.1016/j.bbalip.2016.03.005
_version_ 1782429223608123392
author Bascoul-Colombo, Cécile
Guschina, Irina A.
Maskrey, Benjamin H.
Good, Mark
O'Donnell, Valerie B.
Harwood, John L.
author_facet Bascoul-Colombo, Cécile
Guschina, Irina A.
Maskrey, Benjamin H.
Good, Mark
O'Donnell, Valerie B.
Harwood, John L.
author_sort Bascoul-Colombo, Cécile
collection PubMed
description Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids and amyloid pathology. Because dietary docosahexaenoic acid (DHA) has been identified as beneficial, we compared mice fed with a DHA-supplemented diet to those on a nutritionally-sufficient diet. Major phospholipids from cortex, hippocampus and cerebellum were separated and analysed. Each phosphoglyceride had a characteristic fatty acid composition which was similar in cortex and hippocampus but different in the cerebellum. The biggest changes on DHA-supplementation were within ethanolamine phospholipids which, together with phosphatidylserine, had the highest proportions of DHA. Reciprocal alterations in DHA and arachidonate were found. The main diet-induced alterations were found in ethanolamine phospholipids, (and included their ether derivatives), as were the changes observed due to genotype. Tg mice appeared more sensitive to diet with generally lower DHA percentages when on the standard diet and higher relative proportions of DHA when the diet was supplemented. All four major phosphoglycerides analysed showed age-dependent decreases in polyunsaturated fatty acid contents. These data provide, for the first time, a detailed evaluation of phospholipids in different brain areas previously shown to be relevant to behaviour in the Tg2576 mouse model for AD. The lipid changes observed with genotype are consistent with the subtle alterations found in AD patients, especially for the ethanolamine phospholipid molecular species. They also emphasise the contrasting changes in fatty acid content induced by DHA supplementation within individual phospholipid classes.
format Online
Article
Text
id pubmed-4847476
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier Pub. Co
record_format MEDLINE/PubMed
spelling pubmed-48474762016-06-01 Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease Bascoul-Colombo, Cécile Guschina, Irina A. Maskrey, Benjamin H. Good, Mark O'Donnell, Valerie B. Harwood, John L. Biochim Biophys Acta Article Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids and amyloid pathology. Because dietary docosahexaenoic acid (DHA) has been identified as beneficial, we compared mice fed with a DHA-supplemented diet to those on a nutritionally-sufficient diet. Major phospholipids from cortex, hippocampus and cerebellum were separated and analysed. Each phosphoglyceride had a characteristic fatty acid composition which was similar in cortex and hippocampus but different in the cerebellum. The biggest changes on DHA-supplementation were within ethanolamine phospholipids which, together with phosphatidylserine, had the highest proportions of DHA. Reciprocal alterations in DHA and arachidonate were found. The main diet-induced alterations were found in ethanolamine phospholipids, (and included their ether derivatives), as were the changes observed due to genotype. Tg mice appeared more sensitive to diet with generally lower DHA percentages when on the standard diet and higher relative proportions of DHA when the diet was supplemented. All four major phosphoglycerides analysed showed age-dependent decreases in polyunsaturated fatty acid contents. These data provide, for the first time, a detailed evaluation of phospholipids in different brain areas previously shown to be relevant to behaviour in the Tg2576 mouse model for AD. The lipid changes observed with genotype are consistent with the subtle alterations found in AD patients, especially for the ethanolamine phospholipid molecular species. They also emphasise the contrasting changes in fatty acid content induced by DHA supplementation within individual phospholipid classes. Elsevier Pub. Co 2016-06 /pmc/articles/PMC4847476/ /pubmed/26968097 http://dx.doi.org/10.1016/j.bbalip.2016.03.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bascoul-Colombo, Cécile
Guschina, Irina A.
Maskrey, Benjamin H.
Good, Mark
O'Donnell, Valerie B.
Harwood, John L.
Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title_full Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title_fullStr Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title_full_unstemmed Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title_short Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease
title_sort dietary dha supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the tg2576 mouse model of alzheimer's disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4847476/
https://www.ncbi.nlm.nih.gov/pubmed/26968097
http://dx.doi.org/10.1016/j.bbalip.2016.03.005
work_keys_str_mv AT bascoulcolombocecile dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease
AT guschinairinaa dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease
AT maskreybenjaminh dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease
AT goodmark dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease
AT odonnellvalerieb dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease
AT harwoodjohnl dietarydhasupplementationcausesselectivechangesinphospholipidsfromdifferentbrainregionsinbothwildtypemiceandthetg2576mousemodelofalzheimersdisease