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Engineering a High-Throughput 3-D In Vitro Glioblastoma Model

Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumor in adults because of its highly invasive behavior. The existing treatment for GBM, which involves a combination of resection, chemotherapy, and radiotherapy, has a very limited success rate with a median survival rate...

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Formato: Online Artículo Texto
Lenguaje:English
Publicado: IEEE 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848064/
https://www.ncbi.nlm.nih.gov/pubmed/27170911
http://dx.doi.org/10.1109/JTEHM.2015.2410277
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description Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumor in adults because of its highly invasive behavior. The existing treatment for GBM, which involves a combination of resection, chemotherapy, and radiotherapy, has a very limited success rate with a median survival rate of <1 year. This is mainly because of the failure of early detection and effective treatment. We designed a novel 3-D GBM cell culture model based on microwells that could mimic in vitro environment and help to bypass the lack of suitable animal models for preclinical toxicity tests. Microwells were fabricated from simple and inexpensive polyethylene glycol material for the control of in vitro 3-D culture. We applied the 3-D micropatterning system to GBM (U-87) cells using the photolithography technique to control the cell spheroids’ shape, size, and thickness. Our preliminary results suggested that uniform GBM spheroids can be formed in 3-D, and the size of these GBM spheroids depends on the size of microwells. The viability of the spheroids generated in this manner was quantitatively evaluated using live/dead assay and shown to improve over 21 days. We believe that in vitro 3-D cell culture model could help to reduce the time of the preclinical brain tumor growth studies. The proposed novel platform could be useful and cost-effective for high-throughput screening of cancer drugs and assessment of treatment responses.
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spelling pubmed-48480642016-05-11 Engineering a High-Throughput 3-D In Vitro Glioblastoma Model IEEE J Transl Eng Health Med Article Glioblastoma multiforme (GBM) is the most common and malignant primary brain tumor in adults because of its highly invasive behavior. The existing treatment for GBM, which involves a combination of resection, chemotherapy, and radiotherapy, has a very limited success rate with a median survival rate of <1 year. This is mainly because of the failure of early detection and effective treatment. We designed a novel 3-D GBM cell culture model based on microwells that could mimic in vitro environment and help to bypass the lack of suitable animal models for preclinical toxicity tests. Microwells were fabricated from simple and inexpensive polyethylene glycol material for the control of in vitro 3-D culture. We applied the 3-D micropatterning system to GBM (U-87) cells using the photolithography technique to control the cell spheroids’ shape, size, and thickness. Our preliminary results suggested that uniform GBM spheroids can be formed in 3-D, and the size of these GBM spheroids depends on the size of microwells. The viability of the spheroids generated in this manner was quantitatively evaluated using live/dead assay and shown to improve over 21 days. We believe that in vitro 3-D cell culture model could help to reduce the time of the preclinical brain tumor growth studies. The proposed novel platform could be useful and cost-effective for high-throughput screening of cancer drugs and assessment of treatment responses. IEEE 2015-03-05 /pmc/articles/PMC4848064/ /pubmed/27170911 http://dx.doi.org/10.1109/JTEHM.2015.2410277 Text en 2168-2372 © 2015 IEEE. Translations and content mining are permitted for academic research only. Personal use is also permitted, but republication/redistribution requires IEEE permission. See http://www.ieee.org/publications_standards/publications/rights/index.html for more information.
spellingShingle Article
Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title_full Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title_fullStr Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title_full_unstemmed Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title_short Engineering a High-Throughput 3-D In Vitro Glioblastoma Model
title_sort engineering a high-throughput 3-d in vitro glioblastoma model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848064/
https://www.ncbi.nlm.nih.gov/pubmed/27170911
http://dx.doi.org/10.1109/JTEHM.2015.2410277
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