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Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice
The embryonic origin of lymphatic endothelial cells (LECs) has been a matter of controversy since more than a century. However, recent studies in mice have supported the concept that embryonic lymphangiogenesis is a complex process consisting of growth of lymphatics from specific venous segments as...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848334/ https://www.ncbi.nlm.nih.gov/pubmed/26748643 http://dx.doi.org/10.1007/s00418-015-1399-y |
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author | Buttler, K. Lohrberg, M. Gross, G. Weich, H. A. Wilting, J. |
author_facet | Buttler, K. Lohrberg, M. Gross, G. Weich, H. A. Wilting, J. |
author_sort | Buttler, K. |
collection | PubMed |
description | The embryonic origin of lymphatic endothelial cells (LECs) has been a matter of controversy since more than a century. However, recent studies in mice have supported the concept that embryonic lymphangiogenesis is a complex process consisting of growth of lymphatics from specific venous segments as well as the integration of lymphangioblasts into the lymphatic networks. Similarly, the mechanisms of adult lymphangiogenesis are poorly understood and have rarely been studied. We have recently shown that endothelial progenitor cells isolated from the lung of adult mice have the capacity to form both blood vessels and lymphatics when grafted with Matrigel plugs into the skin of syngeneic mice. Here, we followed up on these experiments and studied the behavior of host leukocytes during lymphangiogenesis in the Matrigel plugs. We observed a striking co-localization of CD45(+) leukocytes with the developing lymphatics. Numerous CD45(+) cells expressed the LEC marker podoplanin and were obviously integrated into the lining of lymphatic capillaries. This indicates that, similar to inflammation-induced lymphangiogenesis in man, circulating CD45(+) cells of adult mice are capable of initiating lymphangiogenesis and of adopting a lymphvasculogenic cellular differentiation program. The data are discussed in the context of embryonic and inflammation-induced lymphangiogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00418-015-1399-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4848334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-48483342016-05-12 Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice Buttler, K. Lohrberg, M. Gross, G. Weich, H. A. Wilting, J. Histochem Cell Biol Original Paper The embryonic origin of lymphatic endothelial cells (LECs) has been a matter of controversy since more than a century. However, recent studies in mice have supported the concept that embryonic lymphangiogenesis is a complex process consisting of growth of lymphatics from specific venous segments as well as the integration of lymphangioblasts into the lymphatic networks. Similarly, the mechanisms of adult lymphangiogenesis are poorly understood and have rarely been studied. We have recently shown that endothelial progenitor cells isolated from the lung of adult mice have the capacity to form both blood vessels and lymphatics when grafted with Matrigel plugs into the skin of syngeneic mice. Here, we followed up on these experiments and studied the behavior of host leukocytes during lymphangiogenesis in the Matrigel plugs. We observed a striking co-localization of CD45(+) leukocytes with the developing lymphatics. Numerous CD45(+) cells expressed the LEC marker podoplanin and were obviously integrated into the lining of lymphatic capillaries. This indicates that, similar to inflammation-induced lymphangiogenesis in man, circulating CD45(+) cells of adult mice are capable of initiating lymphangiogenesis and of adopting a lymphvasculogenic cellular differentiation program. The data are discussed in the context of embryonic and inflammation-induced lymphangiogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00418-015-1399-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-01-09 2016 /pmc/articles/PMC4848334/ /pubmed/26748643 http://dx.doi.org/10.1007/s00418-015-1399-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Buttler, K. Lohrberg, M. Gross, G. Weich, H. A. Wilting, J. Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title | Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title_full | Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title_fullStr | Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title_full_unstemmed | Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title_short | Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice |
title_sort | integration of cd45-positive leukocytes into newly forming lymphatics of adult mice |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848334/ https://www.ncbi.nlm.nih.gov/pubmed/26748643 http://dx.doi.org/10.1007/s00418-015-1399-y |
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