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MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells

C-type natriuretic peptide (CNP) plays a role as an oocyte maturation inhibitor (OMI) in many species, including the bovine. However, the effects of luteinizing hormone (LH) on CNP expression and its potential mechanisms have not reported in the bovine. In the present study, we aimed to study the ef...

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Autores principales: YANG, Lei, WEI, Qiang, GE, Junbang, ZHAO, Xiaoe, MA, Baohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848570/
https://www.ncbi.nlm.nih.gov/pubmed/26655567
http://dx.doi.org/10.1262/jrd.2015-135
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author YANG, Lei
WEI, Qiang
GE, Junbang
ZHAO, Xiaoe
MA, Baohua
author_facet YANG, Lei
WEI, Qiang
GE, Junbang
ZHAO, Xiaoe
MA, Baohua
author_sort YANG, Lei
collection PubMed
description C-type natriuretic peptide (CNP) plays a role as an oocyte maturation inhibitor (OMI) in many species, including the bovine. However, the effects of luteinizing hormone (LH) on CNP expression and its potential mechanisms have not reported in the bovine. In the present study, we aimed to study the effects of LH on CNP expression and to illuminate the potential molecular mechanism in this process. Our results showed that LH induced epidermal growth factor receptor (EGFR) phosphorylation, mitogen-activated protein kinase3/1 (MAPK3/1) activation and CNP mRNA decrease in cultured bovine granulosa cells. Further study revealed that LH suppressed CNP expression via the MAPK3/1 signaling pathway, which was activated by the EGFR pathway. In conclusion, our research suggested that MAPK3/1 is involved in LH-mediated decrease of CNP and that this process is related to the EGFR and MAPK3/1 signal pathways.
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spelling pubmed-48485702016-04-28 MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells YANG, Lei WEI, Qiang GE, Junbang ZHAO, Xiaoe MA, Baohua J Reprod Dev Original Article C-type natriuretic peptide (CNP) plays a role as an oocyte maturation inhibitor (OMI) in many species, including the bovine. However, the effects of luteinizing hormone (LH) on CNP expression and its potential mechanisms have not reported in the bovine. In the present study, we aimed to study the effects of LH on CNP expression and to illuminate the potential molecular mechanism in this process. Our results showed that LH induced epidermal growth factor receptor (EGFR) phosphorylation, mitogen-activated protein kinase3/1 (MAPK3/1) activation and CNP mRNA decrease in cultured bovine granulosa cells. Further study revealed that LH suppressed CNP expression via the MAPK3/1 signaling pathway, which was activated by the EGFR pathway. In conclusion, our research suggested that MAPK3/1 is involved in LH-mediated decrease of CNP and that this process is related to the EGFR and MAPK3/1 signal pathways. The Society for Reproduction and Development 2015-12-10 2016-04 /pmc/articles/PMC4848570/ /pubmed/26655567 http://dx.doi.org/10.1262/jrd.2015-135 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
YANG, Lei
WEI, Qiang
GE, Junbang
ZHAO, Xiaoe
MA, Baohua
MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title_full MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title_fullStr MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title_full_unstemmed MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title_short MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells
title_sort mapk3/1 is conducive to luteinizing hormone-mediated c-type natriuretic peptide decrease in bovine granulosa cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848570/
https://www.ncbi.nlm.nih.gov/pubmed/26655567
http://dx.doi.org/10.1262/jrd.2015-135
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