Cargando…
N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation
Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). Th...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848705/ https://www.ncbi.nlm.nih.gov/pubmed/27110821 http://dx.doi.org/10.3390/nu8040237 |
_version_ | 1782429401196003328 |
---|---|
author | Zhang, Li Tian, Feng Gao, Xuejin Wang, Xinying Wu, Chao Li, Ning Li, Jieshou |
author_facet | Zhang, Li Tian, Feng Gao, Xuejin Wang, Xinying Wu, Chao Li, Ning Li, Jieshou |
author_sort | Zhang, Li |
collection | PubMed |
description | Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). The sham group did not undergo surgery, while mice in the remaining groups were sacrificed 1–5 days after hemorrhagic shock/resuscitation. In the second part, mice were treated with saline or fish oil (n = 8 per group) five days after injury. We determined serum triglyceride levels and liver tissues were collected and prepared for qRT-PCR or Western blot analysis. We found that triglyceride levels were increased five days after hemorrhagic shock/resuscitation, but decreased after addition of fish oil. After injury, the protein and gene expression of carnitine palmitoyltransferase 1A, fatty acid transport protein 1, and peroxisome proliferator-activated receptor-α decreased significantly in liver tissue. In contrast, after treatment with fish oil, the expression levels of these targets increased compared with those in the saline group. The present results suggest n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. This function is possibly accomplished through activating the peroxisome proliferator-activated receptor-α pathway. |
format | Online Article Text |
id | pubmed-4848705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48487052016-05-04 N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation Zhang, Li Tian, Feng Gao, Xuejin Wang, Xinying Wu, Chao Li, Ning Li, Jieshou Nutrients Article Appropriate metabolic interventions after hemorrhagic shock/resuscitation injury have not yet been identified. We aimed to examine the effects of fish oil on lipid metabolic intervention after hemorrhagic shock/resuscitation. Firstly, 48 C57BL/6 mice were assigned to six groups (n = 8 per group). The sham group did not undergo surgery, while mice in the remaining groups were sacrificed 1–5 days after hemorrhagic shock/resuscitation. In the second part, mice were treated with saline or fish oil (n = 8 per group) five days after injury. We determined serum triglyceride levels and liver tissues were collected and prepared for qRT-PCR or Western blot analysis. We found that triglyceride levels were increased five days after hemorrhagic shock/resuscitation, but decreased after addition of fish oil. After injury, the protein and gene expression of carnitine palmitoyltransferase 1A, fatty acid transport protein 1, and peroxisome proliferator-activated receptor-α decreased significantly in liver tissue. In contrast, after treatment with fish oil, the expression levels of these targets increased compared with those in the saline group. The present results suggest n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. This function is possibly accomplished through activating the peroxisome proliferator-activated receptor-α pathway. MDPI 2016-04-22 /pmc/articles/PMC4848705/ /pubmed/27110821 http://dx.doi.org/10.3390/nu8040237 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Li Tian, Feng Gao, Xuejin Wang, Xinying Wu, Chao Li, Ning Li, Jieshou N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title | N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title_full | N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title_fullStr | N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title_full_unstemmed | N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title_short | N-3 Polyunsaturated Fatty Acids Improve Liver Lipid Oxidation-Related Enzyme Levels and Increased the Peroxisome Proliferator-Activated Receptor α Expression Level in Mice Subjected to Hemorrhagic Shock/Resuscitation |
title_sort | n-3 polyunsaturated fatty acids improve liver lipid oxidation-related enzyme levels and increased the peroxisome proliferator-activated receptor α expression level in mice subjected to hemorrhagic shock/resuscitation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848705/ https://www.ncbi.nlm.nih.gov/pubmed/27110821 http://dx.doi.org/10.3390/nu8040237 |
work_keys_str_mv | AT zhangli n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT tianfeng n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT gaoxuejin n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT wangxinying n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT wuchao n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT lining n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation AT lijieshou n3polyunsaturatedfattyacidsimproveliverlipidoxidationrelatedenzymelevelsandincreasedtheperoxisomeproliferatoractivatedreceptoraexpressionlevelinmicesubjectedtohemorrhagicshockresuscitation |