MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland

Depletion of Brca1 leads to defects in mouse mammary gland development and mammary tumors in humans and mice. To explore the role of microRNAs (miRNAs) in this process, we examined the mammary glands of MMTV-Cre Brca1(Co/Co) mice for differential miRNA expression using a candidate approach. Several...

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Autores principales: Wronski, A, Sandhu, G K, Milevskiy, M J G, Brewster, B L, Bridge, J A, Shewan, A M, Edwards, S L, French, J D, Brown, M A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848838/
https://www.ncbi.nlm.nih.gov/pubmed/27043663
http://dx.doi.org/10.1038/oncsis.2016.27
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author Wronski, A
Sandhu, G K
Milevskiy, M J G
Brewster, B L
Bridge, J A
Shewan, A M
Edwards, S L
French, J D
Brown, M A
author_facet Wronski, A
Sandhu, G K
Milevskiy, M J G
Brewster, B L
Bridge, J A
Shewan, A M
Edwards, S L
French, J D
Brown, M A
author_sort Wronski, A
collection PubMed
description Depletion of Brca1 leads to defects in mouse mammary gland development and mammary tumors in humans and mice. To explore the role of microRNAs (miRNAs) in this process, we examined the mammary glands of MMTV-Cre Brca1(Co/Co) mice for differential miRNA expression using a candidate approach. Several miRNAs were differentially expressed in mammary tissue at day 1 of lactation and in mammary epithelial cell lines in which Brca1 messenger RNA (mRNA) levels have been reduced. Functional studies revealed that several of these miRNAs regulate mammary epithelial cell function in vitro, including miR-206. Creation and analysis of MMTV-miR-206 transgenic mice showed no effect on lactational mammary development and no tumors, but indicates a role in mammary tissue remodeling in mature mice, potentially involving Igf-1 and Sfrp1. These results indicate the potential of miRNAs to mediate the consequences of Brca1 loss and suggest a novel function for miR-206.
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spelling pubmed-48488382016-05-09 MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland Wronski, A Sandhu, G K Milevskiy, M J G Brewster, B L Bridge, J A Shewan, A M Edwards, S L French, J D Brown, M A Oncogenesis Short Communication Depletion of Brca1 leads to defects in mouse mammary gland development and mammary tumors in humans and mice. To explore the role of microRNAs (miRNAs) in this process, we examined the mammary glands of MMTV-Cre Brca1(Co/Co) mice for differential miRNA expression using a candidate approach. Several miRNAs were differentially expressed in mammary tissue at day 1 of lactation and in mammary epithelial cell lines in which Brca1 messenger RNA (mRNA) levels have been reduced. Functional studies revealed that several of these miRNAs regulate mammary epithelial cell function in vitro, including miR-206. Creation and analysis of MMTV-miR-206 transgenic mice showed no effect on lactational mammary development and no tumors, but indicates a role in mammary tissue remodeling in mature mice, potentially involving Igf-1 and Sfrp1. These results indicate the potential of miRNAs to mediate the consequences of Brca1 loss and suggest a novel function for miR-206. Nature Publishing Group 2016-04 2016-04-04 /pmc/articles/PMC4848838/ /pubmed/27043663 http://dx.doi.org/10.1038/oncsis.2016.27 Text en Copyright © 2016 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Short Communication
Wronski, A
Sandhu, G K
Milevskiy, M J G
Brewster, B L
Bridge, J A
Shewan, A M
Edwards, S L
French, J D
Brown, M A
MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title_full MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title_fullStr MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title_full_unstemmed MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title_short MicroRNA-206 is differentially expressed in Brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
title_sort microrna-206 is differentially expressed in brca1-deficient mice and regulates epithelial and stromal cell compartments of the mouse mammary gland
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848838/
https://www.ncbi.nlm.nih.gov/pubmed/27043663
http://dx.doi.org/10.1038/oncsis.2016.27
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