Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway

Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the...

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Detalles Bibliográficos
Autores principales: Damalakiene, Leona, Karabanovas, Vitalijus, Bagdonas, Saulius, Rotomskis, Ricardas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848929/
http://dx.doi.org/10.3390/ijms17040473
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author Damalakiene, Leona
Karabanovas, Vitalijus
Bagdonas, Saulius
Rotomskis, Ricardas
author_facet Damalakiene, Leona
Karabanovas, Vitalijus
Bagdonas, Saulius
Rotomskis, Ricardas
author_sort Damalakiene, Leona
collection PubMed
description Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the intracellular QDs in different intracellular microenvironment, which revealed structural heterogeneity of endosomes and enabled the distinguishing among endosomes of different maturity.
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spelling pubmed-48489292016-05-04 Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway Damalakiene, Leona Karabanovas, Vitalijus Bagdonas, Saulius Rotomskis, Ricardas Int J Mol Sci Communication Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the intracellular QDs in different intracellular microenvironment, which revealed structural heterogeneity of endosomes and enabled the distinguishing among endosomes of different maturity. MDPI 2016-03-30 /pmc/articles/PMC4848929/ http://dx.doi.org/10.3390/ijms17040473 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Damalakiene, Leona
Karabanovas, Vitalijus
Bagdonas, Saulius
Rotomskis, Ricardas
Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title_full Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title_fullStr Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title_full_unstemmed Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title_short Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway
title_sort fluorescence-lifetime imaging microscopy for visualization of quantum dots’ endocytic pathway
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848929/
http://dx.doi.org/10.3390/ijms17040473
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AT bagdonassaulius fluorescencelifetimeimagingmicroscopyforvisualizationofquantumdotsendocyticpathway
AT rotomskisricardas fluorescencelifetimeimagingmicroscopyforvisualizationofquantumdotsendocyticpathway

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