Cargando…

Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway

Quinine is a bitter tasting compound that is involved in the regulation of body weight as demonstrated in in vivo animal models and in vitro models of the adipogenic system. Arguments exist over the positive or negative roles of quinine in both in vivo animal models and in vitro cell models, which m...

Descripción completa

Detalles Bibliográficos
Autores principales: Ning, Xiaomin, He, Jingjing, Shi, Xin’e, Yang, Gongshe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848960/
https://www.ncbi.nlm.nih.gov/pubmed/27089323
http://dx.doi.org/10.3390/ijms17040504
_version_ 1782429457443717120
author Ning, Xiaomin
He, Jingjing
Shi, Xin’e
Yang, Gongshe
author_facet Ning, Xiaomin
He, Jingjing
Shi, Xin’e
Yang, Gongshe
author_sort Ning, Xiaomin
collection PubMed
description Quinine is a bitter tasting compound that is involved in the regulation of body weight as demonstrated in in vivo animal models and in vitro models of the adipogenic system. Arguments exist over the positive or negative roles of quinine in both in vivo animal models and in vitro cell models, which motivates us to further investigate the functions of quinine in the in vitro adipogenic system. To clarify the regulatory functions of quinine in adipogenesis, mouse primary preadipocytes were induced for differentiation with quinine supplementation. The results showed that quinine enhanced adipogenesis in a dose dependent manner without affecting lipolysis. The pro-adipogenic effect of quinine was specific, as other bitter tasting agonists had no effect on adipogenesis. Moreover, the pro-adipogenic effect of quinine was mediated by activation of ERK/S6 (extracellular-signal-regulated kinase/Ribosomal protein S6) signaling. Knockdown of bitter taste receptor T2R106 (taste receptor, type 2, member 106) impaired the pro-adipogenic effect of quinine and suppressed the activation of ERK/S6 signaling. Taken together, quinine stimulates adipogenesis through ERK/S6 signaling, which at least partly functions via T2R106.
format Online
Article
Text
id pubmed-4848960
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48489602016-05-04 Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway Ning, Xiaomin He, Jingjing Shi, Xin’e Yang, Gongshe Int J Mol Sci Article Quinine is a bitter tasting compound that is involved in the regulation of body weight as demonstrated in in vivo animal models and in vitro models of the adipogenic system. Arguments exist over the positive or negative roles of quinine in both in vivo animal models and in vitro cell models, which motivates us to further investigate the functions of quinine in the in vitro adipogenic system. To clarify the regulatory functions of quinine in adipogenesis, mouse primary preadipocytes were induced for differentiation with quinine supplementation. The results showed that quinine enhanced adipogenesis in a dose dependent manner without affecting lipolysis. The pro-adipogenic effect of quinine was specific, as other bitter tasting agonists had no effect on adipogenesis. Moreover, the pro-adipogenic effect of quinine was mediated by activation of ERK/S6 (extracellular-signal-regulated kinase/Ribosomal protein S6) signaling. Knockdown of bitter taste receptor T2R106 (taste receptor, type 2, member 106) impaired the pro-adipogenic effect of quinine and suppressed the activation of ERK/S6 signaling. Taken together, quinine stimulates adipogenesis through ERK/S6 signaling, which at least partly functions via T2R106. MDPI 2016-04-13 /pmc/articles/PMC4848960/ /pubmed/27089323 http://dx.doi.org/10.3390/ijms17040504 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ning, Xiaomin
He, Jingjing
Shi, Xin’e
Yang, Gongshe
Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title_full Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title_fullStr Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title_full_unstemmed Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title_short Regulation of Adipogenesis by Quinine through the ERK/S6 Pathway
title_sort regulation of adipogenesis by quinine through the erk/s6 pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848960/
https://www.ncbi.nlm.nih.gov/pubmed/27089323
http://dx.doi.org/10.3390/ijms17040504
work_keys_str_mv AT ningxiaomin regulationofadipogenesisbyquininethroughtheerks6pathway
AT hejingjing regulationofadipogenesisbyquininethroughtheerks6pathway
AT shixine regulationofadipogenesisbyquininethroughtheerks6pathway
AT yanggongshe regulationofadipogenesisbyquininethroughtheerks6pathway