Cargando…
Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR)
Galectins (gal) are members of the mammalian β-galactoside-binding proteins and recognize Galβ1-4GlcNAc and Galβ1-4GalNac (Thomsen-Friedenreich antigen (TF)) sequences of several cell surface oligosaccharides. In this study, gal-1, -2, -3 and -13 were investigated systematically in the trophoblast a...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848979/ https://www.ncbi.nlm.nih.gov/pubmed/27070577 http://dx.doi.org/10.3390/ijms17040523 |
_version_ | 1782429461823619072 |
---|---|
author | Hutter, Stefan Knabl, Julia Andergassen, Ulrich Hofmann, Simone Kuhn, Christina Mahner, Sven Arck, Petra Jeschke, Udo |
author_facet | Hutter, Stefan Knabl, Julia Andergassen, Ulrich Hofmann, Simone Kuhn, Christina Mahner, Sven Arck, Petra Jeschke, Udo |
author_sort | Hutter, Stefan |
collection | PubMed |
description | Galectins (gal) are members of the mammalian β-galactoside-binding proteins and recognize Galβ1-4GlcNAc and Galβ1-4GalNac (Thomsen-Friedenreich antigen (TF)) sequences of several cell surface oligosaccharides. In this study, gal-1, -2, -3 and -13 were investigated systematically in the trophoblast and decidua compartment of intrauterine growth restriction (IUGR) placentas and normal third trimester control placentas and stratified by fetal gender and gestational age. Within this study, 29 third trimester placentas after delivery were analyzed. Fetal gender was equally divided within both groups, and immunohistochemical staining was analyzed according to fetal gender and gestational age. Double immune-fluorescence with trophoblast-specific markers was used to identify galectin-expressing cells at the feto-maternal interface in the decidua. Gal-3 was significantly downregulated only in the extravillous trophoblast of IUGR placentas. In contrast, expressions of gal-2 and gal-13 were downregulated in both villous and extravillous trophoblast cells of IUGR placentas. In addition, gal-2 and gal-13 showed a highly correlated expression scheme in the placenta. There are significant gender-specific expression patterns for single prototype galectins with downregulation of gal-2 and gal-13 of male gender placentas in cases of IUGR. Gal-3 as the chimera type galectin shows only little gender-specific differences in expression, which disappear in IUGR cases. |
format | Online Article Text |
id | pubmed-4848979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48489792016-05-04 Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) Hutter, Stefan Knabl, Julia Andergassen, Ulrich Hofmann, Simone Kuhn, Christina Mahner, Sven Arck, Petra Jeschke, Udo Int J Mol Sci Article Galectins (gal) are members of the mammalian β-galactoside-binding proteins and recognize Galβ1-4GlcNAc and Galβ1-4GalNac (Thomsen-Friedenreich antigen (TF)) sequences of several cell surface oligosaccharides. In this study, gal-1, -2, -3 and -13 were investigated systematically in the trophoblast and decidua compartment of intrauterine growth restriction (IUGR) placentas and normal third trimester control placentas and stratified by fetal gender and gestational age. Within this study, 29 third trimester placentas after delivery were analyzed. Fetal gender was equally divided within both groups, and immunohistochemical staining was analyzed according to fetal gender and gestational age. Double immune-fluorescence with trophoblast-specific markers was used to identify galectin-expressing cells at the feto-maternal interface in the decidua. Gal-3 was significantly downregulated only in the extravillous trophoblast of IUGR placentas. In contrast, expressions of gal-2 and gal-13 were downregulated in both villous and extravillous trophoblast cells of IUGR placentas. In addition, gal-2 and gal-13 showed a highly correlated expression scheme in the placenta. There are significant gender-specific expression patterns for single prototype galectins with downregulation of gal-2 and gal-13 of male gender placentas in cases of IUGR. Gal-3 as the chimera type galectin shows only little gender-specific differences in expression, which disappear in IUGR cases. MDPI 2016-04-07 /pmc/articles/PMC4848979/ /pubmed/27070577 http://dx.doi.org/10.3390/ijms17040523 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hutter, Stefan Knabl, Julia Andergassen, Ulrich Hofmann, Simone Kuhn, Christina Mahner, Sven Arck, Petra Jeschke, Udo Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title | Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title_full | Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title_fullStr | Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title_full_unstemmed | Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title_short | Placental Expression Patterns of Galectin-1, Galectin-2, Galectin-3 and Galectin-13 in Cases of Intrauterine Growth Restriction (IUGR) |
title_sort | placental expression patterns of galectin-1, galectin-2, galectin-3 and galectin-13 in cases of intrauterine growth restriction (iugr) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848979/ https://www.ncbi.nlm.nih.gov/pubmed/27070577 http://dx.doi.org/10.3390/ijms17040523 |
work_keys_str_mv | AT hutterstefan placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT knabljulia placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT andergassenulrich placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT hofmannsimone placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT kuhnchristina placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT mahnersven placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT arckpetra placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr AT jeschkeudo placentalexpressionpatternsofgalectin1galectin2galectin3andgalectin13incasesofintrauterinegrowthrestrictioniugr |