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The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines

Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and w...

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Autores principales: Liu, Wen-Sheng, Ma, Jing-E, Li, Wei-Xia, Zhang, Jin-Ge, Wang, Juan, Nie, Qing-Hua, Qiu, Feng-Fang, Fang, Mei-Xia, Zeng, Fang, Wang, Xing, Lin, Xi-Ran, Zhang, Li, Chen, Shao-Hao, Zhang, Xi-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848999/
https://www.ncbi.nlm.nih.gov/pubmed/27077853
http://dx.doi.org/10.3390/ijms17040543
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author Liu, Wen-Sheng
Ma, Jing-E
Li, Wei-Xia
Zhang, Jin-Ge
Wang, Juan
Nie, Qing-Hua
Qiu, Feng-Fang
Fang, Mei-Xia
Zeng, Fang
Wang, Xing
Lin, Xi-Ran
Zhang, Li
Chen, Shao-Hao
Zhang, Xi-Quan
author_facet Liu, Wen-Sheng
Ma, Jing-E
Li, Wei-Xia
Zhang, Jin-Ge
Wang, Juan
Nie, Qing-Hua
Qiu, Feng-Fang
Fang, Mei-Xia
Zeng, Fang
Wang, Xing
Lin, Xi-Ran
Zhang, Li
Chen, Shao-Hao
Zhang, Xi-Quan
author_sort Liu, Wen-Sheng
collection PubMed
description Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle’s GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves’ turtle might correlate with downregulated gene expression.
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spelling pubmed-48489992016-05-04 The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines Liu, Wen-Sheng Ma, Jing-E Li, Wei-Xia Zhang, Jin-Ge Wang, Juan Nie, Qing-Hua Qiu, Feng-Fang Fang, Mei-Xia Zeng, Fang Wang, Xing Lin, Xi-Ran Zhang, Li Chen, Shao-Hao Zhang, Xi-Quan Int J Mol Sci Article Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle’s GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves’ turtle might correlate with downregulated gene expression. MDPI 2016-04-12 /pmc/articles/PMC4848999/ /pubmed/27077853 http://dx.doi.org/10.3390/ijms17040543 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Wen-Sheng
Ma, Jing-E
Li, Wei-Xia
Zhang, Jin-Ge
Wang, Juan
Nie, Qing-Hua
Qiu, Feng-Fang
Fang, Mei-Xia
Zeng, Fang
Wang, Xing
Lin, Xi-Ran
Zhang, Li
Chen, Shao-Hao
Zhang, Xi-Quan
The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title_full The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title_fullStr The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title_full_unstemmed The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title_short The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
title_sort long intron 1 of growth hormone gene from reeves’ turtle (chinemys reevesii) correlates with negatively regulated gh expression in four cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848999/
https://www.ncbi.nlm.nih.gov/pubmed/27077853
http://dx.doi.org/10.3390/ijms17040543
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