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The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines
Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and w...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848999/ https://www.ncbi.nlm.nih.gov/pubmed/27077853 http://dx.doi.org/10.3390/ijms17040543 |
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author | Liu, Wen-Sheng Ma, Jing-E Li, Wei-Xia Zhang, Jin-Ge Wang, Juan Nie, Qing-Hua Qiu, Feng-Fang Fang, Mei-Xia Zeng, Fang Wang, Xing Lin, Xi-Ran Zhang, Li Chen, Shao-Hao Zhang, Xi-Quan |
author_facet | Liu, Wen-Sheng Ma, Jing-E Li, Wei-Xia Zhang, Jin-Ge Wang, Juan Nie, Qing-Hua Qiu, Feng-Fang Fang, Mei-Xia Zeng, Fang Wang, Xing Lin, Xi-Ran Zhang, Li Chen, Shao-Hao Zhang, Xi-Quan |
author_sort | Liu, Wen-Sheng |
collection | PubMed |
description | Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle’s GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves’ turtle might correlate with downregulated gene expression. |
format | Online Article Text |
id | pubmed-4848999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48489992016-05-04 The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines Liu, Wen-Sheng Ma, Jing-E Li, Wei-Xia Zhang, Jin-Ge Wang, Juan Nie, Qing-Hua Qiu, Feng-Fang Fang, Mei-Xia Zeng, Fang Wang, Xing Lin, Xi-Ran Zhang, Li Chen, Shao-Hao Zhang, Xi-Quan Int J Mol Sci Article Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle’s GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves’ turtle might correlate with downregulated gene expression. MDPI 2016-04-12 /pmc/articles/PMC4848999/ /pubmed/27077853 http://dx.doi.org/10.3390/ijms17040543 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Wen-Sheng Ma, Jing-E Li, Wei-Xia Zhang, Jin-Ge Wang, Juan Nie, Qing-Hua Qiu, Feng-Fang Fang, Mei-Xia Zeng, Fang Wang, Xing Lin, Xi-Ran Zhang, Li Chen, Shao-Hao Zhang, Xi-Quan The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title | The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title_full | The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title_fullStr | The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title_full_unstemmed | The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title_short | The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines |
title_sort | long intron 1 of growth hormone gene from reeves’ turtle (chinemys reevesii) correlates with negatively regulated gh expression in four cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4848999/ https://www.ncbi.nlm.nih.gov/pubmed/27077853 http://dx.doi.org/10.3390/ijms17040543 |
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