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Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken
miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In thi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849015/ https://www.ncbi.nlm.nih.gov/pubmed/27089330 http://dx.doi.org/10.3390/ijms17040559 |
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author | Jia, Xinzheng Lin, Huiran Abdalla, Bahareldin Ali Nie, Qinghua |
author_facet | Jia, Xinzheng Lin, Huiran Abdalla, Bahareldin Ali Nie, Qinghua |
author_sort | Jia, Xinzheng |
collection | PubMed |
description | miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In this region, many key transcription factors, including MyoD, c-Myb, CEBPα/β, AP-4, RAP1, Brn2, GATA-1/2/3, E47, Sn, upstream stimulatory factor (USF) and CdxA, were predicted to bind and interact with miR-206 promoter. Overexpression of MyoD sharply increased miR-206 expression in both fibroblast and myoblast cells, and also the regulation in the myoblast cells was much stronger, indicating that miR-206 was regulated by MyoD combined with other muscle specific transcriptional factors. Aiming to further investigate the relationship between miR-206 mutation and transcriptional expression, total of 23 SNPs were identified in the two distinct bird lines by sequencing. Interestingly, the motif bound by MyoD was individually destroyed by G-to-C mutation located at 419 bp upstream of miR-206 precursor. Co-transfecting MyoD and miR-206 promoter in DF-1 cells, the luciferase activity of promoter containing homozygous GG types was significantly higher than CC ones (p < 0.05). Thus, this mutation caused low expression of miR-206. Consistently, eight variants including G-419C mutation exhibited a great effect on birthweight through maker-trait association analysis in F2 population (p < 0.05). Additionally, the regulation of miR-206 on embryo muscle mass mainly by increasing MyoG and muscle creatine kinase (MCK) expression (p < 0.05) with little change in MyoD, TMEM8C and myosin heavy chain (MHC). In conclusion, our findings provide a novel mutation destroying the promoter activity of miR-206 in birds and shed new light to understand the regulation mechanism of miR-206 on the embryonic muscle growth. |
format | Online Article Text |
id | pubmed-4849015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48490152016-05-04 Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken Jia, Xinzheng Lin, Huiran Abdalla, Bahareldin Ali Nie, Qinghua Int J Mol Sci Article miRNAs have been widely investigated in terms of cell proliferation and differentiation. However, little is known about their effects on bird growth. Here we characterized the promoter of miR-206 in chicken and found that the preferable promoter was located in 1200 bp upstream of pri-miR-206. In this region, many key transcription factors, including MyoD, c-Myb, CEBPα/β, AP-4, RAP1, Brn2, GATA-1/2/3, E47, Sn, upstream stimulatory factor (USF) and CdxA, were predicted to bind and interact with miR-206 promoter. Overexpression of MyoD sharply increased miR-206 expression in both fibroblast and myoblast cells, and also the regulation in the myoblast cells was much stronger, indicating that miR-206 was regulated by MyoD combined with other muscle specific transcriptional factors. Aiming to further investigate the relationship between miR-206 mutation and transcriptional expression, total of 23 SNPs were identified in the two distinct bird lines by sequencing. Interestingly, the motif bound by MyoD was individually destroyed by G-to-C mutation located at 419 bp upstream of miR-206 precursor. Co-transfecting MyoD and miR-206 promoter in DF-1 cells, the luciferase activity of promoter containing homozygous GG types was significantly higher than CC ones (p < 0.05). Thus, this mutation caused low expression of miR-206. Consistently, eight variants including G-419C mutation exhibited a great effect on birthweight through maker-trait association analysis in F2 population (p < 0.05). Additionally, the regulation of miR-206 on embryo muscle mass mainly by increasing MyoG and muscle creatine kinase (MCK) expression (p < 0.05) with little change in MyoD, TMEM8C and myosin heavy chain (MHC). In conclusion, our findings provide a novel mutation destroying the promoter activity of miR-206 in birds and shed new light to understand the regulation mechanism of miR-206 on the embryonic muscle growth. MDPI 2016-04-14 /pmc/articles/PMC4849015/ /pubmed/27089330 http://dx.doi.org/10.3390/ijms17040559 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jia, Xinzheng Lin, Huiran Abdalla, Bahareldin Ali Nie, Qinghua Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title | Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title_full | Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title_fullStr | Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title_full_unstemmed | Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title_short | Characterization of miR-206 Promoter and Its Association with Birthweight in Chicken |
title_sort | characterization of mir-206 promoter and its association with birthweight in chicken |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849015/ https://www.ncbi.nlm.nih.gov/pubmed/27089330 http://dx.doi.org/10.3390/ijms17040559 |
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