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Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases
In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS) for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowin...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849058/ https://www.ncbi.nlm.nih.gov/pubmed/27110774 http://dx.doi.org/10.3390/ijms17040607 |
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author | Antonucci, Ivana Provenzano, Martina Rodrigues, Melissa Pantalone, Andrea Salini, Vincenzo Ballerini, Patrizia Borlongan, Cesar V. Stuppia, Liborio |
author_facet | Antonucci, Ivana Provenzano, Martina Rodrigues, Melissa Pantalone, Andrea Salini, Vincenzo Ballerini, Patrizia Borlongan, Cesar V. Stuppia, Liborio |
author_sort | Antonucci, Ivana |
collection | PubMed |
description | In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS) for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowing investigations into the molecular mechanisms of the disease. However, the protocol of iPS generation still suffers from technical limitations, showing low efficiency, being expensive and time consuming. Amniotic Fluid Stem cells (AFS) represent a potential alternative novel source of stem cells for modeling of human genetic diseases. In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. This can provide a useful stem cell model for the investigation of the molecular basis of the diagnosed disease without the necessity of producing iPS, since AFS cells show some features of pluripotency and are able to differentiate in cells derived from all three germ layers “in vitro”. In this article, we describe the potential benefits provided by using AFS cells in the modeling of human genetic diseases. |
format | Online Article Text |
id | pubmed-4849058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48490582016-05-04 Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases Antonucci, Ivana Provenzano, Martina Rodrigues, Melissa Pantalone, Andrea Salini, Vincenzo Ballerini, Patrizia Borlongan, Cesar V. Stuppia, Liborio Int J Mol Sci Review In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS) for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowing investigations into the molecular mechanisms of the disease. However, the protocol of iPS generation still suffers from technical limitations, showing low efficiency, being expensive and time consuming. Amniotic Fluid Stem cells (AFS) represent a potential alternative novel source of stem cells for modeling of human genetic diseases. In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. This can provide a useful stem cell model for the investigation of the molecular basis of the diagnosed disease without the necessity of producing iPS, since AFS cells show some features of pluripotency and are able to differentiate in cells derived from all three germ layers “in vitro”. In this article, we describe the potential benefits provided by using AFS cells in the modeling of human genetic diseases. MDPI 2016-04-22 /pmc/articles/PMC4849058/ /pubmed/27110774 http://dx.doi.org/10.3390/ijms17040607 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Antonucci, Ivana Provenzano, Martina Rodrigues, Melissa Pantalone, Andrea Salini, Vincenzo Ballerini, Patrizia Borlongan, Cesar V. Stuppia, Liborio Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title | Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title_full | Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title_fullStr | Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title_full_unstemmed | Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title_short | Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases |
title_sort | amniotic fluid stem cells: a novel source for modeling of human genetic diseases |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849058/ https://www.ncbi.nlm.nih.gov/pubmed/27110774 http://dx.doi.org/10.3390/ijms17040607 |
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