Cargando…

Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46

Breast cancer is the second leading cause of cancer death in women and the presence of metastasis significantly decreases survival. MicroRNAs are involved in tumor progression and the metastatic spreading of breast cancer. Here, we reported that a microRNA, mmu-miR-1894, significantly decreased the...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Li, Li, Xiaoying, Dong, Wei, Sun, Caixian, Guo, Deyu, Zhang, Lianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849059/
https://www.ncbi.nlm.nih.gov/pubmed/27110773
http://dx.doi.org/10.3390/ijms17040609
_version_ 1782429480186281984
author Zhang, Li
Li, Xiaoying
Dong, Wei
Sun, Caixian
Guo, Deyu
Zhang, Lianfeng
author_facet Zhang, Li
Li, Xiaoying
Dong, Wei
Sun, Caixian
Guo, Deyu
Zhang, Lianfeng
author_sort Zhang, Li
collection PubMed
description Breast cancer is the second leading cause of cancer death in women and the presence of metastasis significantly decreases survival. MicroRNAs are involved in tumor progression and the metastatic spreading of breast cancer. Here, we reported that a microRNA, mmu-miR-1894, significantly decreased the lung metastasis of 4TO7 mouse breast cancer cells by 86.7% in mouse models. Mmu-miR-1894-3p was the functional mature form of miR-1894 and significantly decreased the lung metastasis of 4TO7 cells by 90.8% in mouse models. A dual-luciferase reporter assay indicated that mmu-miR-1894-3p directly targeted the tripartite motif containing 46 (Trim46) 3′-untranslated region (UTR) and downregulated the expression of Trim46 in 4TO7 cells. Consistent with the effect of mmu-miR-1894-3p, knockdown of Trim46 inhibited the experimental lung metastasis of 4TO7 cells. Moreover, knockdown of human Trim46 also prohibited the cell proliferation, migration and wound healing of MBA-MD-231 human breast cancer cells. These results suggested that the effect of knockdown of Trim46 alone was sufficient to recapitulate the effect of mmu-miR-1894 on the metastasis of the breast cancer cells in mouse and that Trim46 was involved in the proliferation and migration of mouse and human breast cancer cells.
format Online
Article
Text
id pubmed-4849059
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48490592016-05-04 Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46 Zhang, Li Li, Xiaoying Dong, Wei Sun, Caixian Guo, Deyu Zhang, Lianfeng Int J Mol Sci Article Breast cancer is the second leading cause of cancer death in women and the presence of metastasis significantly decreases survival. MicroRNAs are involved in tumor progression and the metastatic spreading of breast cancer. Here, we reported that a microRNA, mmu-miR-1894, significantly decreased the lung metastasis of 4TO7 mouse breast cancer cells by 86.7% in mouse models. Mmu-miR-1894-3p was the functional mature form of miR-1894 and significantly decreased the lung metastasis of 4TO7 cells by 90.8% in mouse models. A dual-luciferase reporter assay indicated that mmu-miR-1894-3p directly targeted the tripartite motif containing 46 (Trim46) 3′-untranslated region (UTR) and downregulated the expression of Trim46 in 4TO7 cells. Consistent with the effect of mmu-miR-1894-3p, knockdown of Trim46 inhibited the experimental lung metastasis of 4TO7 cells. Moreover, knockdown of human Trim46 also prohibited the cell proliferation, migration and wound healing of MBA-MD-231 human breast cancer cells. These results suggested that the effect of knockdown of Trim46 alone was sufficient to recapitulate the effect of mmu-miR-1894 on the metastasis of the breast cancer cells in mouse and that Trim46 was involved in the proliferation and migration of mouse and human breast cancer cells. MDPI 2016-04-22 /pmc/articles/PMC4849059/ /pubmed/27110773 http://dx.doi.org/10.3390/ijms17040609 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Li
Li, Xiaoying
Dong, Wei
Sun, Caixian
Guo, Deyu
Zhang, Lianfeng
Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title_full Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title_fullStr Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title_full_unstemmed Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title_short Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
title_sort mmu-mir-1894-3p inhibits cell proliferation and migration of breast cancer cells by targeting trim46
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849059/
https://www.ncbi.nlm.nih.gov/pubmed/27110773
http://dx.doi.org/10.3390/ijms17040609
work_keys_str_mv AT zhangli mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46
AT lixiaoying mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46
AT dongwei mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46
AT suncaixian mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46
AT guodeyu mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46
AT zhanglianfeng mmumir18943pinhibitscellproliferationandmigrationofbreastcancercellsbytargetingtrim46