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Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium

Animal cell cytokinesis results from patterned activation of the small GTPase Rho, which directs assembly of actomyosin in the equatorial cortex. Cytokinesis is restricted to a portion of the cell cycle following anaphase onset in which the cortex is responsive to signals from the spindle. We show t...

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Autores principales: Bement, William M., Leda, Marcin, Moe, Alison M., Kita, Angela M., Larson, Matthew E., Golding, Adriana E., Pfeuti, Courtney, Su, Kuan-Chung, Miller, Ann L., Goryachev, Andrew B., von Dassow, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849138/
https://www.ncbi.nlm.nih.gov/pubmed/26479320
http://dx.doi.org/10.1038/ncb3251
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author Bement, William M.
Leda, Marcin
Moe, Alison M.
Kita, Angela M.
Larson, Matthew E.
Golding, Adriana E.
Pfeuti, Courtney
Su, Kuan-Chung
Miller, Ann L.
Goryachev, Andrew B.
von Dassow, George
author_facet Bement, William M.
Leda, Marcin
Moe, Alison M.
Kita, Angela M.
Larson, Matthew E.
Golding, Adriana E.
Pfeuti, Courtney
Su, Kuan-Chung
Miller, Ann L.
Goryachev, Andrew B.
von Dassow, George
author_sort Bement, William M.
collection PubMed
description Animal cell cytokinesis results from patterned activation of the small GTPase Rho, which directs assembly of actomyosin in the equatorial cortex. Cytokinesis is restricted to a portion of the cell cycle following anaphase onset in which the cortex is responsive to signals from the spindle. We show that shortly after anaphase onset oocytes and embryonic cells of frogs and echinoderms exhibit cortical waves of Rho activity and F-actin polymerization. The waves are modulated by cyclin-dependent kinase 1 (Cdk1) activity and require the Rho GEF (guanine nucleotide exchange factor), Ect2. Surprisingly, during wave propagation, while Rho activity elicits F-actin assembly, F-actin subsequently inactivates Rho. Experimental and modeling results show that waves represent excitable dynamics of a reaction diffusion system with Rho as the activator and F-actin the inhibitor. We propose that cortical excitability explains fundamental features of cytokinesis including its cell cycle regulation.
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spelling pubmed-48491382016-05-01 Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium Bement, William M. Leda, Marcin Moe, Alison M. Kita, Angela M. Larson, Matthew E. Golding, Adriana E. Pfeuti, Courtney Su, Kuan-Chung Miller, Ann L. Goryachev, Andrew B. von Dassow, George Nat Cell Biol Article Animal cell cytokinesis results from patterned activation of the small GTPase Rho, which directs assembly of actomyosin in the equatorial cortex. Cytokinesis is restricted to a portion of the cell cycle following anaphase onset in which the cortex is responsive to signals from the spindle. We show that shortly after anaphase onset oocytes and embryonic cells of frogs and echinoderms exhibit cortical waves of Rho activity and F-actin polymerization. The waves are modulated by cyclin-dependent kinase 1 (Cdk1) activity and require the Rho GEF (guanine nucleotide exchange factor), Ect2. Surprisingly, during wave propagation, while Rho activity elicits F-actin assembly, F-actin subsequently inactivates Rho. Experimental and modeling results show that waves represent excitable dynamics of a reaction diffusion system with Rho as the activator and F-actin the inhibitor. We propose that cortical excitability explains fundamental features of cytokinesis including its cell cycle regulation. 2015-10-19 2015-11 /pmc/articles/PMC4849138/ /pubmed/26479320 http://dx.doi.org/10.1038/ncb3251 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Bement, William M.
Leda, Marcin
Moe, Alison M.
Kita, Angela M.
Larson, Matthew E.
Golding, Adriana E.
Pfeuti, Courtney
Su, Kuan-Chung
Miller, Ann L.
Goryachev, Andrew B.
von Dassow, George
Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title_full Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title_fullStr Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title_full_unstemmed Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title_short Activator-inhibitor coupling between Rho signaling and actin assembly make the cell cortex an excitable medium
title_sort activator-inhibitor coupling between rho signaling and actin assembly make the cell cortex an excitable medium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849138/
https://www.ncbi.nlm.nih.gov/pubmed/26479320
http://dx.doi.org/10.1038/ncb3251
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