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Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows
In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849651/ https://www.ncbi.nlm.nih.gov/pubmed/27124615 http://dx.doi.org/10.1371/journal.pone.0154642 |
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author | Kasparovska, Jitka Pecinkova, Martina Dadakova, Katerina Krizova, Ludmila Hadrova, Sylvie Lexa, Matej Lochman, Jan Kasparovsky, Tomas |
author_facet | Kasparovska, Jitka Pecinkova, Martina Dadakova, Katerina Krizova, Ludmila Hadrova, Sylvie Lexa, Matej Lochman, Jan Kasparovsky, Tomas |
author_sort | Kasparovska, Jitka |
collection | PubMed |
description | In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 μg/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 μg/L in the CTRL group to 186.30 μg/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings. |
format | Online Article Text |
id | pubmed-4849651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48496512016-05-07 Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows Kasparovska, Jitka Pecinkova, Martina Dadakova, Katerina Krizova, Ludmila Hadrova, Sylvie Lexa, Matej Lochman, Jan Kasparovsky, Tomas PLoS One Research Article In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 μg/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 μg/L in the CTRL group to 186.30 μg/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings. Public Library of Science 2016-04-28 /pmc/articles/PMC4849651/ /pubmed/27124615 http://dx.doi.org/10.1371/journal.pone.0154642 Text en © 2016 Kasparovska et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kasparovska, Jitka Pecinkova, Martina Dadakova, Katerina Krizova, Ludmila Hadrova, Sylvie Lexa, Matej Lochman, Jan Kasparovsky, Tomas Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title | Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title_full | Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title_fullStr | Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title_full_unstemmed | Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title_short | Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows |
title_sort | effects of isoflavone-enriched feed on the rumen microbiota in dairy cows |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849651/ https://www.ncbi.nlm.nih.gov/pubmed/27124615 http://dx.doi.org/10.1371/journal.pone.0154642 |
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