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Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome

BACKGROUND/AIMS: Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understa...

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Autores principales: Liang, Wen-Jing, Zhang, Guo, Luo, He-Sheng, Liang, Lie-Xin, Huang, Dan, Zhang, Fa-Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Office of Gut and Liver 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849691/
https://www.ncbi.nlm.nih.gov/pubmed/26446924
http://dx.doi.org/10.5009/gnl14319
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author Liang, Wen-Jing
Zhang, Guo
Luo, He-Sheng
Liang, Lie-Xin
Huang, Dan
Zhang, Fa-Can
author_facet Liang, Wen-Jing
Zhang, Guo
Luo, He-Sheng
Liang, Lie-Xin
Huang, Dan
Zhang, Fa-Can
author_sort Liang, Wen-Jing
collection PubMed
description BACKGROUND/AIMS: Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS. METHODS: Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining. RESULTS: Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only. CONCLUSIONS: Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.
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spelling pubmed-48496912016-05-04 Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome Liang, Wen-Jing Zhang, Guo Luo, He-Sheng Liang, Lie-Xin Huang, Dan Zhang, Fa-Can Gut Liver Original Article BACKGROUND/AIMS: Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS. METHODS: Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining. RESULTS: Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only. CONCLUSIONS: Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS. Editorial Office of Gut and Liver 2016-05 2015-10-12 /pmc/articles/PMC4849691/ /pubmed/26446924 http://dx.doi.org/10.5009/gnl14319 Text en Copyright © 2016 by The Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, Korean College of Helicobacter and Upper Gastrointestinal Research, Korean Association the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, Korean Pancreatobiliary Association, and Korean Society of Gastrointestinal Cancer. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Liang, Wen-Jing
Zhang, Guo
Luo, He-Sheng
Liang, Lie-Xin
Huang, Dan
Zhang, Fa-Can
Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title_full Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title_fullStr Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title_full_unstemmed Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title_short Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
title_sort tryptase and protease-activated receptor 2 expression levels in irritable bowel syndrome
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849691/
https://www.ncbi.nlm.nih.gov/pubmed/26446924
http://dx.doi.org/10.5009/gnl14319
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