Cargando…

Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of liver damage, ranging from simple steatosis to cirrhosis. However, simple steatosis (SS) and steatohepatitis (NASH) cannot yet be distinguished by clinical or laboratory features. The aim of this study was to assess the...

Descripción completa

Detalles Bibliográficos
Autores principales: Aragonès, Gemma, Auguet, Teresa, Berlanga, Alba, Guiu-Jurado, Esther, Martinez, Salomé, Armengol, Sandra, Sabench, Fàtima, Ras, Rosa, Hernandez, Mercè, Aguilar, Carmen, Colom, Josep, Sirvent, Joan Josep, Del Castillo, Daniel, Richart, Cristóbal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849715/
https://www.ncbi.nlm.nih.gov/pubmed/27123846
http://dx.doi.org/10.1371/journal.pone.0154601
_version_ 1782429585183342592
author Aragonès, Gemma
Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Martinez, Salomé
Armengol, Sandra
Sabench, Fàtima
Ras, Rosa
Hernandez, Mercè
Aguilar, Carmen
Colom, Josep
Sirvent, Joan Josep
Del Castillo, Daniel
Richart, Cristóbal
author_facet Aragonès, Gemma
Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Martinez, Salomé
Armengol, Sandra
Sabench, Fàtima
Ras, Rosa
Hernandez, Mercè
Aguilar, Carmen
Colom, Josep
Sirvent, Joan Josep
Del Castillo, Daniel
Richart, Cristóbal
author_sort Aragonès, Gemma
collection PubMed
description BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of liver damage, ranging from simple steatosis to cirrhosis. However, simple steatosis (SS) and steatohepatitis (NASH) cannot yet be distinguished by clinical or laboratory features. The aim of this study was to assess the relationship between alpha-ketoglutarate and the degrees of NAFLD in morbidly obese patients. MATERIALS AND METHODS: We used a gas chromatography-quadruple time-of-flight-mass spectrometry analysis to quantify alpha-ketoglutarate in serum from normal-weight subjects (n = 30) and morbidly obese women (n = 97) with or without NAFLD. RESULTS: We found that serum levels of alpha-ketoglutarate were significantly higher in morbidly obese women than in normal-weight women. We showed that circulating levels of alpha-ketoglutarate were lower in lean controls and morbidly obese patients without NAFLD. We also found that alpha-ketoglutarate serum levels were higher in both SS and NASH than in normal liver of morbidly obese patients. However, there was no difference between SS and NASH. Moreover, we observed that circulating levels of alpha-ketoglutarate were associated with glucose metabolism parameters, lipid profile, hepatic enzymes and steatosis degree. In addition, diagnostic performance of alpha-ketoglutarate has been analyzed in NAFLD patients. The AUROC curves from patients with liver steatosis exhibited an acceptable clinical utility. Finally, we showed that the combination of biomarkers (AST, ALT and alpha-ketoglutarate) had the highest accuracy in diagnosing liver steatosis. CONCLUSION: These findings suggest that alpha-ketoglutarate can determine the presence of non-alcoholic fatty liver in morbidly obese patients but it is not valid a biomarker for NASH.
format Online
Article
Text
id pubmed-4849715
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48497152016-05-07 Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease Aragonès, Gemma Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Martinez, Salomé Armengol, Sandra Sabench, Fàtima Ras, Rosa Hernandez, Mercè Aguilar, Carmen Colom, Josep Sirvent, Joan Josep Del Castillo, Daniel Richart, Cristóbal PLoS One Research Article BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of liver damage, ranging from simple steatosis to cirrhosis. However, simple steatosis (SS) and steatohepatitis (NASH) cannot yet be distinguished by clinical or laboratory features. The aim of this study was to assess the relationship between alpha-ketoglutarate and the degrees of NAFLD in morbidly obese patients. MATERIALS AND METHODS: We used a gas chromatography-quadruple time-of-flight-mass spectrometry analysis to quantify alpha-ketoglutarate in serum from normal-weight subjects (n = 30) and morbidly obese women (n = 97) with or without NAFLD. RESULTS: We found that serum levels of alpha-ketoglutarate were significantly higher in morbidly obese women than in normal-weight women. We showed that circulating levels of alpha-ketoglutarate were lower in lean controls and morbidly obese patients without NAFLD. We also found that alpha-ketoglutarate serum levels were higher in both SS and NASH than in normal liver of morbidly obese patients. However, there was no difference between SS and NASH. Moreover, we observed that circulating levels of alpha-ketoglutarate were associated with glucose metabolism parameters, lipid profile, hepatic enzymes and steatosis degree. In addition, diagnostic performance of alpha-ketoglutarate has been analyzed in NAFLD patients. The AUROC curves from patients with liver steatosis exhibited an acceptable clinical utility. Finally, we showed that the combination of biomarkers (AST, ALT and alpha-ketoglutarate) had the highest accuracy in diagnosing liver steatosis. CONCLUSION: These findings suggest that alpha-ketoglutarate can determine the presence of non-alcoholic fatty liver in morbidly obese patients but it is not valid a biomarker for NASH. Public Library of Science 2016-04-28 /pmc/articles/PMC4849715/ /pubmed/27123846 http://dx.doi.org/10.1371/journal.pone.0154601 Text en © 2016 Aragonès et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aragonès, Gemma
Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Martinez, Salomé
Armengol, Sandra
Sabench, Fàtima
Ras, Rosa
Hernandez, Mercè
Aguilar, Carmen
Colom, Josep
Sirvent, Joan Josep
Del Castillo, Daniel
Richart, Cristóbal
Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title_full Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title_fullStr Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title_full_unstemmed Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title_short Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease
title_sort increased circulating levels of alpha-ketoglutarate in morbidly obese women with non-alcoholic fatty liver disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4849715/
https://www.ncbi.nlm.nih.gov/pubmed/27123846
http://dx.doi.org/10.1371/journal.pone.0154601
work_keys_str_mv AT aragonesgemma increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT auguetteresa increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT berlangaalba increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT guiujuradoesther increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT martinezsalome increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT armengolsandra increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT sabenchfatima increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT rasrosa increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT hernandezmerce increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT aguilarcarmen increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT colomjosep increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT sirventjoanjosep increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT delcastillodaniel increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease
AT richartcristobal increasedcirculatinglevelsofalphaketoglutarateinmorbidlyobesewomenwithnonalcoholicfattyliverdisease