Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)(1)

PREMISE OF THE STUDY: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami’s urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae). METHODS AND RESULTS: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (H(o)) of 0.314 and average expected heterozygosity (H(e)) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average H(o) of 0.35 and an average H(e) of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average H(o) of 0.257 and an average H(e) of 0.272. CONCLUSIONS: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project.