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Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1)
PREMISE OF THE STUDY: Microsatellite markers can be used to evaluate population structure and genetic diversity in native populations of Indigofera pseudotinctoria (Fabaceae) and assess genetic disturbance caused by nonnative plants of the same species. METHODS AND RESULTS: We developed 14 markers f...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850052/ https://www.ncbi.nlm.nih.gov/pubmed/27144104 http://dx.doi.org/10.3732/apps.1500110 |
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author | Otao, Tomoko Kobayashi, Tatsuaki Uehara, Koichi |
author_facet | Otao, Tomoko Kobayashi, Tatsuaki Uehara, Koichi |
author_sort | Otao, Tomoko |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite markers can be used to evaluate population structure and genetic diversity in native populations of Indigofera pseudotinctoria (Fabaceae) and assess genetic disturbance caused by nonnative plants of the same species. METHODS AND RESULTS: We developed 14 markers for I. pseudotinctoria using next-generation sequencing and applied them to test two native populations, totaling 77 individuals, and a transplanted population, imported from a foreign country, of 17 individuals. The mean number of alleles was 3.310, observed heterozygosity was 0.242, and expected heterozygosity was 0.346. The fixation index in the transplanted population was 0.469, which was higher than in the native populations (0.154 and 0.158). In addition, the transplanted population contains one allele that is not shared by the native population. CONCLUSIONS: Microsatellite markers can be useful for evaluating genetic diversity within and between populations and for studying population genetics in I. pseudotinctoria and related species. |
format | Online Article Text |
id | pubmed-4850052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-48500522016-05-03 Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) Otao, Tomoko Kobayashi, Tatsuaki Uehara, Koichi Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite markers can be used to evaluate population structure and genetic diversity in native populations of Indigofera pseudotinctoria (Fabaceae) and assess genetic disturbance caused by nonnative plants of the same species. METHODS AND RESULTS: We developed 14 markers for I. pseudotinctoria using next-generation sequencing and applied them to test two native populations, totaling 77 individuals, and a transplanted population, imported from a foreign country, of 17 individuals. The mean number of alleles was 3.310, observed heterozygosity was 0.242, and expected heterozygosity was 0.346. The fixation index in the transplanted population was 0.469, which was higher than in the native populations (0.154 and 0.158). In addition, the transplanted population contains one allele that is not shared by the native population. CONCLUSIONS: Microsatellite markers can be useful for evaluating genetic diversity within and between populations and for studying population genetics in I. pseudotinctoria and related species. Botanical Society of America 2016-04-18 /pmc/articles/PMC4850052/ /pubmed/27144104 http://dx.doi.org/10.3732/apps.1500110 Text en © 2016 Otao et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Otao, Tomoko Kobayashi, Tatsuaki Uehara, Koichi Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title | Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title_full | Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title_fullStr | Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title_full_unstemmed | Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title_short | Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)(1) |
title_sort | development and characterization of 14 microsatellite markers for indigofera pseudotinctoria (fabaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850052/ https://www.ncbi.nlm.nih.gov/pubmed/27144104 http://dx.doi.org/10.3732/apps.1500110 |
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