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Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1)
PREMISE OF THE STUDY: Microsatellites were developed for Astragalus bibullatus (Fabaceae), a federally endangered narrow endemic, to investigate reproductive ecology and species boundaries among closely related taxa. METHODS AND RESULTS: Next-generation sequencing was used to develop 12 nuclear micr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850055/ https://www.ncbi.nlm.nih.gov/pubmed/27144107 http://dx.doi.org/10.3732/apps.1500126 |
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author | Morris, Ashley B. Scalf, Cassandra Burleyson, Austin Johnson, La Tonya Trostel, Kevin |
author_facet | Morris, Ashley B. Scalf, Cassandra Burleyson, Austin Johnson, La Tonya Trostel, Kevin |
author_sort | Morris, Ashley B. |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellites were developed for Astragalus bibullatus (Fabaceae), a federally endangered narrow endemic, to investigate reproductive ecology and species boundaries among closely related taxa. METHODS AND RESULTS: Next-generation sequencing was used to develop 12 nuclear microsatellite loci that amplify in A. bibullatus, as well as in A. crassicarpus var. trichocalyx, A. gypsodes, and A. tennesseensis. Identified loci were di- and trinucleotide repeats, with 1–15 alleles per locus. Observed and expected heterozygosities ranged from 0.000–0.938 and 0.000–0.860, respectively. Cross-amplification of three loci previously published in A. michauxii was also confirmed for the taxa included here. CONCLUSIONS: These data indicate the utility of novel microsatellite loci for conservation genetics and reproductive ecology in closely related Astragalus species. |
format | Online Article Text |
id | pubmed-4850055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-48500552016-05-03 Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) Morris, Ashley B. Scalf, Cassandra Burleyson, Austin Johnson, La Tonya Trostel, Kevin Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellites were developed for Astragalus bibullatus (Fabaceae), a federally endangered narrow endemic, to investigate reproductive ecology and species boundaries among closely related taxa. METHODS AND RESULTS: Next-generation sequencing was used to develop 12 nuclear microsatellite loci that amplify in A. bibullatus, as well as in A. crassicarpus var. trichocalyx, A. gypsodes, and A. tennesseensis. Identified loci were di- and trinucleotide repeats, with 1–15 alleles per locus. Observed and expected heterozygosities ranged from 0.000–0.938 and 0.000–0.860, respectively. Cross-amplification of three loci previously published in A. michauxii was also confirmed for the taxa included here. CONCLUSIONS: These data indicate the utility of novel microsatellite loci for conservation genetics and reproductive ecology in closely related Astragalus species. Botanical Society of America 2016-04-05 /pmc/articles/PMC4850055/ /pubmed/27144107 http://dx.doi.org/10.3732/apps.1500126 Text en © 2016 Morris et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Morris, Ashley B. Scalf, Cassandra Burleyson, Austin Johnson, La Tonya Trostel, Kevin Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title | Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title_full | Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title_fullStr | Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title_full_unstemmed | Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title_short | Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)(1) |
title_sort | development and characterization of microsatellite primers in the federally endangered astragalus bibullatus (fabaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850055/ https://www.ncbi.nlm.nih.gov/pubmed/27144107 http://dx.doi.org/10.3732/apps.1500126 |
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