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Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation

The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording techniq...

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Autores principales: Liu, Chi Chi, Li, Li, Lam, Yun Wah, Siu, Chung Wah, Cheng, Shuk Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850402/
https://www.ncbi.nlm.nih.gov/pubmed/27125643
http://dx.doi.org/10.1038/srep25073
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author Liu, Chi Chi
Li, Li
Lam, Yun Wah
Siu, Chung Wah
Cheng, Shuk Han
author_facet Liu, Chi Chi
Li, Li
Lam, Yun Wah
Siu, Chung Wah
Cheng, Shuk Han
author_sort Liu, Chi Chi
collection PubMed
description The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording technique was improved using a multi-electrode method and by pre-opening the pericardial sac. A convenient ECG data analysis method without wavelet transform was also established. Intraperitoneal injection of KCl in zebrafish induced an arrhythmia similar to that of humans, and the arrhythmia was partially rescued by calcium gluconate. Amputation and cryoinjury of the zebrafish heart induced ST segment depression and affected QRS duration after injury. Only cryoinjury decelerated the heart rate. Different changes were also observed in the QT interval during heart regeneration in these two injury models. We also characterized the electrocardiophysiology of breakdance zebrafish mutant with a prolonged QT interval, that has not been well described in previous studies. Our study provided a reliable and reproducible means to record zebrafish ECG and analyse data. The detailed characterization of the cardiac electrophysiology of zebrafish and its mutant revealed that the potential of the zebrafish in modeling the human cardiovascular system exceeds expectations.
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spelling pubmed-48504022016-05-05 Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation Liu, Chi Chi Li, Li Lam, Yun Wah Siu, Chung Wah Cheng, Shuk Han Sci Rep Article The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording technique was improved using a multi-electrode method and by pre-opening the pericardial sac. A convenient ECG data analysis method without wavelet transform was also established. Intraperitoneal injection of KCl in zebrafish induced an arrhythmia similar to that of humans, and the arrhythmia was partially rescued by calcium gluconate. Amputation and cryoinjury of the zebrafish heart induced ST segment depression and affected QRS duration after injury. Only cryoinjury decelerated the heart rate. Different changes were also observed in the QT interval during heart regeneration in these two injury models. We also characterized the electrocardiophysiology of breakdance zebrafish mutant with a prolonged QT interval, that has not been well described in previous studies. Our study provided a reliable and reproducible means to record zebrafish ECG and analyse data. The detailed characterization of the cardiac electrophysiology of zebrafish and its mutant revealed that the potential of the zebrafish in modeling the human cardiovascular system exceeds expectations. Nature Publishing Group 2016-04-29 /pmc/articles/PMC4850402/ /pubmed/27125643 http://dx.doi.org/10.1038/srep25073 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Chi Chi
Li, Li
Lam, Yun Wah
Siu, Chung Wah
Cheng, Shuk Han
Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title_full Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title_fullStr Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title_full_unstemmed Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title_short Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation
title_sort improvement of surface ecg recording in adult zebrafish reveals that the value of this model exceeds our expectation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850402/
https://www.ncbi.nlm.nih.gov/pubmed/27125643
http://dx.doi.org/10.1038/srep25073
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