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The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate

Bi-phasic or diauxic growth is often observed when microbes are grown in a chemically defined medium containing two sugars (for example glucose and lactose). Typically, the two growth stages are separated by an often lengthy phase of arrested growth, the so-called lag-phase. Diauxic growth is usuall...

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Autores principales: Chu, Dominique, Barnes, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850433/
https://www.ncbi.nlm.nih.gov/pubmed/27125900
http://dx.doi.org/10.1038/srep25191
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author Chu, Dominique
Barnes, David J.
author_facet Chu, Dominique
Barnes, David J.
author_sort Chu, Dominique
collection PubMed
description Bi-phasic or diauxic growth is often observed when microbes are grown in a chemically defined medium containing two sugars (for example glucose and lactose). Typically, the two growth stages are separated by an often lengthy phase of arrested growth, the so-called lag-phase. Diauxic growth is usually interpreted as an adaptation to maximise population growth in multi-nutrient environments. However, the lag-phase implies a substantial loss of growth during the switch-over. It therefore remains unexplained why the lag-phase is adaptive. Here we show by means of a stochastic simulation model based on the bacterial PTS system that it is not possible to shorten the lag-phase without incurring a permanent growth-penalty. Mechanistically, this is due to the inherent and well established limitations of biological sensors to operate efficiently at a given resource cost. Hence, there is a trade-off between lost growth during the diauxic switch and the long-term growth potential of the cell. Using simulated evolution we predict that the lag-phase will evolve depending on the distribution of conditions experienced during adaptation. In environments where switching is less frequently required, the lag-phase will evolve to be longer whereas, in frequently changing environments, the lag-phase will evolve to be shorter.
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spelling pubmed-48504332016-05-05 The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate Chu, Dominique Barnes, David J. Sci Rep Article Bi-phasic or diauxic growth is often observed when microbes are grown in a chemically defined medium containing two sugars (for example glucose and lactose). Typically, the two growth stages are separated by an often lengthy phase of arrested growth, the so-called lag-phase. Diauxic growth is usually interpreted as an adaptation to maximise population growth in multi-nutrient environments. However, the lag-phase implies a substantial loss of growth during the switch-over. It therefore remains unexplained why the lag-phase is adaptive. Here we show by means of a stochastic simulation model based on the bacterial PTS system that it is not possible to shorten the lag-phase without incurring a permanent growth-penalty. Mechanistically, this is due to the inherent and well established limitations of biological sensors to operate efficiently at a given resource cost. Hence, there is a trade-off between lost growth during the diauxic switch and the long-term growth potential of the cell. Using simulated evolution we predict that the lag-phase will evolve depending on the distribution of conditions experienced during adaptation. In environments where switching is less frequently required, the lag-phase will evolve to be longer whereas, in frequently changing environments, the lag-phase will evolve to be shorter. Nature Publishing Group 2016-04-29 /pmc/articles/PMC4850433/ /pubmed/27125900 http://dx.doi.org/10.1038/srep25191 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chu, Dominique
Barnes, David J.
The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title_full The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title_fullStr The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title_full_unstemmed The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title_short The lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
title_sort lag-phase during diauxic growth is a trade-off between fast adaptation and high growth rate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850433/
https://www.ncbi.nlm.nih.gov/pubmed/27125900
http://dx.doi.org/10.1038/srep25191
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