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The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background

BACKGROUND: The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant...

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Autores principales: Zhang, Hui, Wang, Shuang, Zhang, Xiang xiang, Ji, Wei, Song, Fuping, Zhao, Yue, Li, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850703/
https://www.ncbi.nlm.nih.gov/pubmed/27125644
http://dx.doi.org/10.1186/s12934-016-0463-1
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author Zhang, Hui
Wang, Shuang
Zhang, Xiang xiang
Ji, Wei
Song, Fuping
Zhao, Yue
Li, Jie
author_facet Zhang, Hui
Wang, Shuang
Zhang, Xiang xiang
Ji, Wei
Song, Fuping
Zhao, Yue
Li, Jie
author_sort Zhang, Hui
collection PubMed
description BACKGROUND: The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins in these recombinant strains still remain, such as alpha-amylase and alpha-glucosidase; lead to a low-purity of fermentation products. The aim was to construct an A. niger host strain with a low background of protein secretion. RESULTS: The transcription factor amyR was deleted in A. niger CICC2462, and the results from enzyme activity assays and SDS-PAGE analysis showed that the glucoamylase and amylase activities of the ∆amyR strains were significantly lower than those of the wild-type strain. High-throughput RNA-sequencing and shotgun LC–MS/MS proteomic technology analysis demonstrated that the expression of amylolytic enzymes was decreased at both the transcriptional and translational levels in the ∆amyR strain. Interestingly, the ∆amyR strain growth rate better than the wild-type strain. CONCLUSIONS: Our findings clearly indicated that the ∆amyR strain of A. niger CICC2462 can be used as a host strain with a low background of protein secretion.
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spelling pubmed-48507032016-04-30 The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background Zhang, Hui Wang, Shuang Zhang, Xiang xiang Ji, Wei Song, Fuping Zhao, Yue Li, Jie Microb Cell Fact Research BACKGROUND: The filamentous fungus Aspergillus niger is widely exploited as an important expression host for industrial production. The glucoamylase high-producing strain A. niger CICC2462 has been used as a host strain for the establishment of a secretion expression system. It expresses recombinant xylanase, mannase and asparaginase at a high level, but some high secretory background proteins in these recombinant strains still remain, such as alpha-amylase and alpha-glucosidase; lead to a low-purity of fermentation products. The aim was to construct an A. niger host strain with a low background of protein secretion. RESULTS: The transcription factor amyR was deleted in A. niger CICC2462, and the results from enzyme activity assays and SDS-PAGE analysis showed that the glucoamylase and amylase activities of the ∆amyR strains were significantly lower than those of the wild-type strain. High-throughput RNA-sequencing and shotgun LC–MS/MS proteomic technology analysis demonstrated that the expression of amylolytic enzymes was decreased at both the transcriptional and translational levels in the ∆amyR strain. Interestingly, the ∆amyR strain growth rate better than the wild-type strain. CONCLUSIONS: Our findings clearly indicated that the ∆amyR strain of A. niger CICC2462 can be used as a host strain with a low background of protein secretion. BioMed Central 2016-04-28 /pmc/articles/PMC4850703/ /pubmed/27125644 http://dx.doi.org/10.1186/s12934-016-0463-1 Text en © Zhang et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Hui
Wang, Shuang
Zhang, Xiang xiang
Ji, Wei
Song, Fuping
Zhao, Yue
Li, Jie
The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title_full The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title_fullStr The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title_full_unstemmed The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title_short The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background
title_sort amyr-deletion strain of aspergillus niger cicc2462 is a suitable host strain to express secreted protein with a low background
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850703/
https://www.ncbi.nlm.nih.gov/pubmed/27125644
http://dx.doi.org/10.1186/s12934-016-0463-1
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