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Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia

BACKGROUND: To develop and evaluate the feasibility of emerging interventions, animal models with accurate anatomical environment are required. OBJECTIVES: We aimed to establish a clinically relevant colorectal tumor model with canine transmissible venereal tumor (CTVT) utilizing endoscopic ultrasou...

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Autores principales: Bhutani, Manoop S., Uthamanthil, Rajesh, Suzuki, Rei, Shetty, Anil, Klumpp, Sherry A., Nau, William, Stafford, Roger Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850800/
https://www.ncbi.nlm.nih.gov/pubmed/27080606
http://dx.doi.org/10.4103/2303-9027.180471
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author Bhutani, Manoop S.
Uthamanthil, Rajesh
Suzuki, Rei
Shetty, Anil
Klumpp, Sherry A.
Nau, William
Stafford, Roger Jason
author_facet Bhutani, Manoop S.
Uthamanthil, Rajesh
Suzuki, Rei
Shetty, Anil
Klumpp, Sherry A.
Nau, William
Stafford, Roger Jason
author_sort Bhutani, Manoop S.
collection PubMed
description BACKGROUND: To develop and evaluate the feasibility of emerging interventions, animal models with accurate anatomical environment are required. OBJECTIVES: We aimed to establish a clinically relevant colorectal tumor model with canine transmissible venereal tumor (CTVT) utilizing endoscopic ultrasound (EUS) imaging guidance. DESIGN: Survival study using a canine model. SETTING: Endoscopic animal research laboratory at a tertiary cancer center. MATERIALS AND METHODS: This study involved five canines. INTERVENTIONS: A colorectal tumor model was established and evaluated in five canines under cyclosporine immune suppression. Under endoscopic imaging guidance, saline was injected into the submucosal layer forming a bleb. Subsequently, CTVT was inoculated into the bleb under EUS guidance. Endoscopy was the primary method of assessing tumor growth. Tumors developed in 60-130 days. Upon detection of lesions >1 cm, the animals were euthanized and the tumors were harvested for histopathological characterization. MAIN OUTCOME MEASUREMENTS: Success rate of tumor growth. The presence or absence of vasculature inside tumors. RESULTS: Colorectal tumor successfully developed in three out of the five animals (60%). Among the ones with tumor growth, average inoculated CTVT volume, incubation time, and tumor size was 1.8 cc, 65.7 days, and 2.0 cm, respectively. The two animals without tumor growth were observed for >100 days. In all the tumors, vascular structure was characterized with CD31 imunohistochemical stain. LIMITATIONS: Small number of animals. CONCLUSION: We succeeded in creating a new colorectal tumor canine model with CTVT utilizing EUS.
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spelling pubmed-48508002016-05-03 Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia Bhutani, Manoop S. Uthamanthil, Rajesh Suzuki, Rei Shetty, Anil Klumpp, Sherry A. Nau, William Stafford, Roger Jason Endosc Ultrasound Original Article BACKGROUND: To develop and evaluate the feasibility of emerging interventions, animal models with accurate anatomical environment are required. OBJECTIVES: We aimed to establish a clinically relevant colorectal tumor model with canine transmissible venereal tumor (CTVT) utilizing endoscopic ultrasound (EUS) imaging guidance. DESIGN: Survival study using a canine model. SETTING: Endoscopic animal research laboratory at a tertiary cancer center. MATERIALS AND METHODS: This study involved five canines. INTERVENTIONS: A colorectal tumor model was established and evaluated in five canines under cyclosporine immune suppression. Under endoscopic imaging guidance, saline was injected into the submucosal layer forming a bleb. Subsequently, CTVT was inoculated into the bleb under EUS guidance. Endoscopy was the primary method of assessing tumor growth. Tumors developed in 60-130 days. Upon detection of lesions >1 cm, the animals were euthanized and the tumors were harvested for histopathological characterization. MAIN OUTCOME MEASUREMENTS: Success rate of tumor growth. The presence or absence of vasculature inside tumors. RESULTS: Colorectal tumor successfully developed in three out of the five animals (60%). Among the ones with tumor growth, average inoculated CTVT volume, incubation time, and tumor size was 1.8 cc, 65.7 days, and 2.0 cm, respectively. The two animals without tumor growth were observed for >100 days. In all the tumors, vascular structure was characterized with CD31 imunohistochemical stain. LIMITATIONS: Small number of animals. CONCLUSION: We succeeded in creating a new colorectal tumor canine model with CTVT utilizing EUS. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4850800/ /pubmed/27080606 http://dx.doi.org/10.4103/2303-9027.180471 Text en Copyright: © 2016 Spring Media Publishing Co. Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Bhutani, Manoop S.
Uthamanthil, Rajesh
Suzuki, Rei
Shetty, Anil
Klumpp, Sherry A.
Nau, William
Stafford, Roger Jason
Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title_full Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title_fullStr Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title_full_unstemmed Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title_short Endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: A large animal model for colon neoplasia
title_sort endoscopic ultrasound-guided inoculation of transmissible venereal tumor in the colon: a large animal model for colon neoplasia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850800/
https://www.ncbi.nlm.nih.gov/pubmed/27080606
http://dx.doi.org/10.4103/2303-9027.180471
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