Current techniques for visualizing RNA in cells

Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have differen...

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Detalles Bibliográficos
Autores principales: Mannack, Lilith V.J.C., Eising, Sebastian, Rentmeister, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2016
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850879/
https://www.ncbi.nlm.nih.gov/pubmed/27158473
http://dx.doi.org/10.12688/f1000research.8151.1
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author Mannack, Lilith V.J.C.
Eising, Sebastian
Rentmeister, Andrea
author_facet Mannack, Lilith V.J.C.
Eising, Sebastian
Rentmeister, Andrea
author_sort Mannack, Lilith V.J.C.
collection PubMed
description Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have different benefits and limitations. This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations.
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spelling pubmed-48508792016-05-06 Current techniques for visualizing RNA in cells Mannack, Lilith V.J.C. Eising, Sebastian Rentmeister, Andrea F1000Res Review Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have different benefits and limitations. This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations. F1000Research 2016-04-28 /pmc/articles/PMC4850879/ /pubmed/27158473 http://dx.doi.org/10.12688/f1000research.8151.1 Text en Copyright: © 2016 Mannack LVJC et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Mannack, Lilith V.J.C.
Eising, Sebastian
Rentmeister, Andrea
Current techniques for visualizing RNA in cells
title Current techniques for visualizing RNA in cells
title_full Current techniques for visualizing RNA in cells
title_fullStr Current techniques for visualizing RNA in cells
title_full_unstemmed Current techniques for visualizing RNA in cells
title_short Current techniques for visualizing RNA in cells
title_sort current techniques for visualizing rna in cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4850879/
https://www.ncbi.nlm.nih.gov/pubmed/27158473
http://dx.doi.org/10.12688/f1000research.8151.1
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