Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1

Serotonin [5-hydroxytryptamine (5-HT)], an inflammatory mediator, contributes to inflammatory pain. The presence of multiple 5-HT subtype receptors on peripheral and central nociceptors complicates the role of 5-HT in pain. Previously, we found that 5-HT(2B/2C) antagonist could block 5-HT-induced me...

Descripción completa

Detalles Bibliográficos
Autores principales: Su, Yeu-Shiuan, Chiu, Yuan-Yi, Lin, Shih-Yuan, Chen, Chih-Cheng, Sun, Wei-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851684/
https://www.ncbi.nlm.nih.gov/pubmed/26635025
http://dx.doi.org/10.1007/s12031-015-0693-4
_version_ 1782429843508428800
author Su, Yeu-Shiuan
Chiu, Yuan-Yi
Lin, Shih-Yuan
Chen, Chih-Cheng
Sun, Wei-Hsin
author_facet Su, Yeu-Shiuan
Chiu, Yuan-Yi
Lin, Shih-Yuan
Chen, Chih-Cheng
Sun, Wei-Hsin
author_sort Su, Yeu-Shiuan
collection PubMed
description Serotonin [5-hydroxytryptamine (5-HT)], an inflammatory mediator, contributes to inflammatory pain. The presence of multiple 5-HT subtype receptors on peripheral and central nociceptors complicates the role of 5-HT in pain. Previously, we found that 5-HT(2B/2C) antagonist could block 5-HT-induced mechanical hyperalgesia. However, the types of neurons or circuits underlying this effect remained unsolved. Here, we demonstrate that the G(q/11)-phospholipase Cβ-protein kinase C(ε) (PKCε) pathway mediated by 5-HT(2B) is involved in 5-HT-induced mechanical hyperalgesia in mice. Administration of a transient receptor potential vanilloid 1 (TRPV1) antagonist inhibited the 5-HT-induced mechanical hyperalgesia. 5-HT injection enhanced 5-HT- and capsaicin-evoked calcium signals specifically in isolectin B(4) (IB(4))-negative neurons; signals were inhibited by a 5-HT(2B/2C) antagonist and PKCε blocker. Thus, 5-HT(2B) mediates 5-HT-induced mechanical hyperalgesia by regulating TRPV1 function.
format Online
Article
Text
id pubmed-4851684
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-48516842016-05-19 Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1 Su, Yeu-Shiuan Chiu, Yuan-Yi Lin, Shih-Yuan Chen, Chih-Cheng Sun, Wei-Hsin J Mol Neurosci Article Serotonin [5-hydroxytryptamine (5-HT)], an inflammatory mediator, contributes to inflammatory pain. The presence of multiple 5-HT subtype receptors on peripheral and central nociceptors complicates the role of 5-HT in pain. Previously, we found that 5-HT(2B/2C) antagonist could block 5-HT-induced mechanical hyperalgesia. However, the types of neurons or circuits underlying this effect remained unsolved. Here, we demonstrate that the G(q/11)-phospholipase Cβ-protein kinase C(ε) (PKCε) pathway mediated by 5-HT(2B) is involved in 5-HT-induced mechanical hyperalgesia in mice. Administration of a transient receptor potential vanilloid 1 (TRPV1) antagonist inhibited the 5-HT-induced mechanical hyperalgesia. 5-HT injection enhanced 5-HT- and capsaicin-evoked calcium signals specifically in isolectin B(4) (IB(4))-negative neurons; signals were inhibited by a 5-HT(2B/2C) antagonist and PKCε blocker. Thus, 5-HT(2B) mediates 5-HT-induced mechanical hyperalgesia by regulating TRPV1 function. Springer US 2015-12-03 2016 /pmc/articles/PMC4851684/ /pubmed/26635025 http://dx.doi.org/10.1007/s12031-015-0693-4 Text en © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Su, Yeu-Shiuan
Chiu, Yuan-Yi
Lin, Shih-Yuan
Chen, Chih-Cheng
Sun, Wei-Hsin
Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title_full Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title_fullStr Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title_full_unstemmed Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title_short Serotonin Receptor 2B Mediates Mechanical Hyperalgesia by Regulating Transient Receptor Potential Vanilloid 1
title_sort serotonin receptor 2b mediates mechanical hyperalgesia by regulating transient receptor potential vanilloid 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851684/
https://www.ncbi.nlm.nih.gov/pubmed/26635025
http://dx.doi.org/10.1007/s12031-015-0693-4
work_keys_str_mv AT suyeushiuan serotoninreceptor2bmediatesmechanicalhyperalgesiabyregulatingtransientreceptorpotentialvanilloid1
AT chiuyuanyi serotoninreceptor2bmediatesmechanicalhyperalgesiabyregulatingtransientreceptorpotentialvanilloid1
AT linshihyuan serotoninreceptor2bmediatesmechanicalhyperalgesiabyregulatingtransientreceptorpotentialvanilloid1
AT chenchihcheng serotoninreceptor2bmediatesmechanicalhyperalgesiabyregulatingtransientreceptorpotentialvanilloid1
AT sunweihsin serotoninreceptor2bmediatesmechanicalhyperalgesiabyregulatingtransientreceptorpotentialvanilloid1

Ejemplares similares