High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets

CRISPR-Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-targets of the broadly used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing only partial or unproven efficacies and other limitations...

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Detalles Bibliográficos
Autores principales: Kleinstiver, Benjamin P., Pattanayak, Vikram, Prew, Michelle S., Tsai, Shengdar Q., Nguyen, Nhu, Zheng, Zongli, Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851738/
https://www.ncbi.nlm.nih.gov/pubmed/26735016
http://dx.doi.org/10.1038/nature16526

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851738/
https://www.ncbi.nlm.nih.gov/pubmed/26735016
http://dx.doi.org/10.1038/nature16526

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