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Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens

BACKGROUND: Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and infer...

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Autores principales: Liu, Shiyou, Wang, Lin, Zheng, Huajun, Xu, Zhixiao, Roellig, Dawn M., Li, Na, Frace, Michael A., Tang, Kevin, Arrowood, Michael J., Moss, Delynn M., Zhang, Longxian, Feng, Yaoyu, Xiao, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851813/
https://www.ncbi.nlm.nih.gov/pubmed/27129308
http://dx.doi.org/10.1186/s12864-016-2632-3
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author Liu, Shiyou
Wang, Lin
Zheng, Huajun
Xu, Zhixiao
Roellig, Dawn M.
Li, Na
Frace, Michael A.
Tang, Kevin
Arrowood, Michael J.
Moss, Delynn M.
Zhang, Longxian
Feng, Yaoyu
Xiao, Lihua
author_facet Liu, Shiyou
Wang, Lin
Zheng, Huajun
Xu, Zhixiao
Roellig, Dawn M.
Li, Na
Frace, Michael A.
Tang, Kevin
Arrowood, Michael J.
Moss, Delynn M.
Zhang, Longxian
Feng, Yaoyu
Xiao, Lihua
author_sort Liu, Shiyou
collection PubMed
description BACKGROUND: Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis. RESULTS: The genome organization, metabolic capabilities and potential invasion mechanism of C. cayetanensis are very similar to those of Eimeria tenella. Propanoyl-CoA degradation, GPI anchor biosynthesis, and N-glycosylation are some apparent metabolic differences between C. cayetanensis and E. tenella. Unlike Eimeria spp., there are no active LTR-retrotransposons identified in C. cayetanensis. The similar repertoire of host cell invasion-related proteins possessed by all coccidia suggests that C. cayetanensis has an invasion process similar to the one in T. gondii and E. tenella. However, the significant reduction in the number of identifiable rhoptry protein kinases, phosphatases and serine protease inhibitors indicates that monoxenous coccidia, especially C. cayetanensis, have limited capabilities or use a different system to regulate host cell nuclear activities. C. cayetanensis does not possess any cluster of genes encoding the TA4-type SAG surface antigens seen in E. tenella, and may use a different family of surface antigens in initial host cell interactions. CONCLUSIONS: Our findings indicate that C. cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. Amino acid metabolism and post-translation modifications of proteins are some major differences between C. cayetanensis and other apicomplexans. The whole genome sequence data of C. cayetanensis improve our understanding of the biology and evolution of this major foodborne pathogen and facilitate the development of intervention measures and advanced diagnostic tools. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2632-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-48518132016-05-01 Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens Liu, Shiyou Wang, Lin Zheng, Huajun Xu, Zhixiao Roellig, Dawn M. Li, Na Frace, Michael A. Tang, Kevin Arrowood, Michael J. Moss, Delynn M. Zhang, Longxian Feng, Yaoyu Xiao, Lihua BMC Genomics Research Article BACKGROUND: Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis. RESULTS: The genome organization, metabolic capabilities and potential invasion mechanism of C. cayetanensis are very similar to those of Eimeria tenella. Propanoyl-CoA degradation, GPI anchor biosynthesis, and N-glycosylation are some apparent metabolic differences between C. cayetanensis and E. tenella. Unlike Eimeria spp., there are no active LTR-retrotransposons identified in C. cayetanensis. The similar repertoire of host cell invasion-related proteins possessed by all coccidia suggests that C. cayetanensis has an invasion process similar to the one in T. gondii and E. tenella. However, the significant reduction in the number of identifiable rhoptry protein kinases, phosphatases and serine protease inhibitors indicates that monoxenous coccidia, especially C. cayetanensis, have limited capabilities or use a different system to regulate host cell nuclear activities. C. cayetanensis does not possess any cluster of genes encoding the TA4-type SAG surface antigens seen in E. tenella, and may use a different family of surface antigens in initial host cell interactions. CONCLUSIONS: Our findings indicate that C. cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. Amino acid metabolism and post-translation modifications of proteins are some major differences between C. cayetanensis and other apicomplexans. The whole genome sequence data of C. cayetanensis improve our understanding of the biology and evolution of this major foodborne pathogen and facilitate the development of intervention measures and advanced diagnostic tools. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2632-3) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-30 /pmc/articles/PMC4851813/ /pubmed/27129308 http://dx.doi.org/10.1186/s12864-016-2632-3 Text en © Liu et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Shiyou
Wang, Lin
Zheng, Huajun
Xu, Zhixiao
Roellig, Dawn M.
Li, Na
Frace, Michael A.
Tang, Kevin
Arrowood, Michael J.
Moss, Delynn M.
Zhang, Longxian
Feng, Yaoyu
Xiao, Lihua
Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title_full Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title_fullStr Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title_full_unstemmed Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title_short Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
title_sort comparative genomics reveals cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851813/
https://www.ncbi.nlm.nih.gov/pubmed/27129308
http://dx.doi.org/10.1186/s12864-016-2632-3
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