Cargando…

Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations

Poly(lactic-co-glycolic acid) (PLGA) chitosan (CS) coated nanoparticles (NPs) were loaded with two antiretrovirals (ARVs) either lamivudine (LMV) which is hydrophilic or nevirapine (NVP) which is hydrophobic or both LMV and NVP. These ARVs are of importance in resource-limited settings, where they a...

Descripción completa

Detalles Bibliográficos
Autores principales: Makita-Chingombe, Faithful, Kutscher, Hilliard L., DiTursi, Sara L., Morse, Gene D., Maponga, Charles C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852115/
https://www.ncbi.nlm.nih.gov/pubmed/27190651
http://dx.doi.org/10.1155/2016/3810175
_version_ 1782429890076737536
author Makita-Chingombe, Faithful
Kutscher, Hilliard L.
DiTursi, Sara L.
Morse, Gene D.
Maponga, Charles C.
author_facet Makita-Chingombe, Faithful
Kutscher, Hilliard L.
DiTursi, Sara L.
Morse, Gene D.
Maponga, Charles C.
author_sort Makita-Chingombe, Faithful
collection PubMed
description Poly(lactic-co-glycolic acid) (PLGA) chitosan (CS) coated nanoparticles (NPs) were loaded with two antiretrovirals (ARVs) either lamivudine (LMV) which is hydrophilic or nevirapine (NVP) which is hydrophobic or both LMV and NVP. These ARVs are of importance in resource-limited settings, where they are commonly used in human immunodeficiency virus (HIV-1) treatment due to affordability and accessibility. NPs prepared by a water-oil-water emulsion and reduced pressure solvent evaporation technique were determined to have a positive zeta potential, a capsule-like morphology, and an average hydrodynamic diameter of 240 nm. Entrapment of NVP as a single ARV had a notable increase in NP size compared to LMV alone or in combination with LMV. NPs stored at room temperature in distilled water maintained size, polydispersity (PDI), and zeta potential for one year. No changes in size, PDI, and zeta potential were observed for NPs in 10% sucrose in lyophilized or nonlyophilized states stored at 4°C and −20°C, respectively. Freezing NPs in the absence of sucrose increased NP size. Drug loading, encapsulation efficiency, and kinetic release profiles were quantified by high performance liquid chromatography (HPLC). Our novel nanoformulations have the potential to improve patient outcomes and expand drug access in resource-limited countries for the treatment of HIV-1.
format Online
Article
Text
id pubmed-4852115
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-48521152016-05-17 Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations Makita-Chingombe, Faithful Kutscher, Hilliard L. DiTursi, Sara L. Morse, Gene D. Maponga, Charles C. J Drug Deliv Research Article Poly(lactic-co-glycolic acid) (PLGA) chitosan (CS) coated nanoparticles (NPs) were loaded with two antiretrovirals (ARVs) either lamivudine (LMV) which is hydrophilic or nevirapine (NVP) which is hydrophobic or both LMV and NVP. These ARVs are of importance in resource-limited settings, where they are commonly used in human immunodeficiency virus (HIV-1) treatment due to affordability and accessibility. NPs prepared by a water-oil-water emulsion and reduced pressure solvent evaporation technique were determined to have a positive zeta potential, a capsule-like morphology, and an average hydrodynamic diameter of 240 nm. Entrapment of NVP as a single ARV had a notable increase in NP size compared to LMV alone or in combination with LMV. NPs stored at room temperature in distilled water maintained size, polydispersity (PDI), and zeta potential for one year. No changes in size, PDI, and zeta potential were observed for NPs in 10% sucrose in lyophilized or nonlyophilized states stored at 4°C and −20°C, respectively. Freezing NPs in the absence of sucrose increased NP size. Drug loading, encapsulation efficiency, and kinetic release profiles were quantified by high performance liquid chromatography (HPLC). Our novel nanoformulations have the potential to improve patient outcomes and expand drug access in resource-limited countries for the treatment of HIV-1. Hindawi Publishing Corporation 2016 2016-04-17 /pmc/articles/PMC4852115/ /pubmed/27190651 http://dx.doi.org/10.1155/2016/3810175 Text en Copyright © 2016 Faithful Makita-Chingombe et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Makita-Chingombe, Faithful
Kutscher, Hilliard L.
DiTursi, Sara L.
Morse, Gene D.
Maponga, Charles C.
Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title_full Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title_fullStr Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title_full_unstemmed Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title_short Poly(lactic-co-glycolic) Acid-Chitosan Dual Loaded Nanoparticles for Antiretroviral Nanoformulations
title_sort poly(lactic-co-glycolic) acid-chitosan dual loaded nanoparticles for antiretroviral nanoformulations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852115/
https://www.ncbi.nlm.nih.gov/pubmed/27190651
http://dx.doi.org/10.1155/2016/3810175
work_keys_str_mv AT makitachingombefaithful polylacticcoglycolicacidchitosandualloadednanoparticlesforantiretroviralnanoformulations
AT kutscherhilliardl polylacticcoglycolicacidchitosandualloadednanoparticlesforantiretroviralnanoformulations
AT ditursisaral polylacticcoglycolicacidchitosandualloadednanoparticlesforantiretroviralnanoformulations
AT morsegened polylacticcoglycolicacidchitosandualloadednanoparticlesforantiretroviralnanoformulations
AT mapongacharlesc polylacticcoglycolicacidchitosandualloadednanoparticlesforantiretroviralnanoformulations