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Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)

Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be e...

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Autores principales: Roth, Caleb C., Glickman, Randolph D., Tolstykh, Gleb P., Estlack, Larry E., Moen, Erick K., Echchgadda, Ibtissam, Beier, Hope T., Barnes, Ronald A., Ibey, Bennett L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852903/
https://www.ncbi.nlm.nih.gov/pubmed/27135944
http://dx.doi.org/10.1371/journal.pone.0154555
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author Roth, Caleb C.
Glickman, Randolph D.
Tolstykh, Gleb P.
Estlack, Larry E.
Moen, Erick K.
Echchgadda, Ibtissam
Beier, Hope T.
Barnes, Ronald A.
Ibey, Bennett L.
author_facet Roth, Caleb C.
Glickman, Randolph D.
Tolstykh, Gleb P.
Estlack, Larry E.
Moen, Erick K.
Echchgadda, Ibtissam
Beier, Hope T.
Barnes, Ronald A.
Ibey, Bennett L.
author_sort Roth, Caleb C.
collection PubMed
description Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.
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spelling pubmed-48529032016-05-13 Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP) Roth, Caleb C. Glickman, Randolph D. Tolstykh, Gleb P. Estlack, Larry E. Moen, Erick K. Echchgadda, Ibtissam Beier, Hope T. Barnes, Ronald A. Ibey, Bennett L. PLoS One Research Article Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress. Public Library of Science 2016-05-02 /pmc/articles/PMC4852903/ /pubmed/27135944 http://dx.doi.org/10.1371/journal.pone.0154555 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Roth, Caleb C.
Glickman, Randolph D.
Tolstykh, Gleb P.
Estlack, Larry E.
Moen, Erick K.
Echchgadda, Ibtissam
Beier, Hope T.
Barnes, Ronald A.
Ibey, Bennett L.
Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title_full Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title_fullStr Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title_full_unstemmed Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title_short Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
title_sort evaluation of the genetic response of u937 and jurkat cells to 10-nanosecond electrical pulses (nsep)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852903/
https://www.ncbi.nlm.nih.gov/pubmed/27135944
http://dx.doi.org/10.1371/journal.pone.0154555
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