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1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens

Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D(3) (1,25D(3)) is well known as a po...

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Autores principales: Zhang, Long, Lu, Lu, Li, Siming, Zhang, Guolong, Ouyang, Linghua, Robinson, Kelsy, Tang, Yanqiang, Zhu, Qing, Li, Diyan, Hu, Yaodong, Liu, Yiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852925/
https://www.ncbi.nlm.nih.gov/pubmed/27135828
http://dx.doi.org/10.1371/journal.pone.0154546
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author Zhang, Long
Lu, Lu
Li, Siming
Zhang, Guolong
Ouyang, Linghua
Robinson, Kelsy
Tang, Yanqiang
Zhu, Qing
Li, Diyan
Hu, Yaodong
Liu, Yiping
author_facet Zhang, Long
Lu, Lu
Li, Siming
Zhang, Guolong
Ouyang, Linghua
Robinson, Kelsy
Tang, Yanqiang
Zhu, Qing
Li, Diyan
Hu, Yaodong
Liu, Yiping
author_sort Zhang, Long
collection PubMed
description Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D(3) (1,25D(3)) is well known as a powerful HDP inducer in humans, but limited information about the effect of 1,25D(3) on HDPs in poultry is available. Here, we sought to examine whether 1,25D(3) could stimulate avian β-defensin (AvBD) expression in chickens. We used chicken embryo intestinal epithelial cells (CEIEPCs) and peripheral blood mononuclear cells (PBMCs) to study the effect of 1,25D(3) on the expression of AvBDs. We observed that 1,25D(3) is able to up-regulate the expression of several AvBDs in CEIEPCs and PBMCs, whereas it increased the amounts of AvBD4 mRNA in CEIEPCs only in the presence of lipopolysaccharide (LPS). On the other hand, LPS treatment not only inhibited the expression of CYP24A1 but also altered the expression pattern of VDR in CEIEPCs. Furthermore, AvBDs were not directly regulated by 1,25D(3), as cycloheximide completely blocked 1,25D(3)-induced expression of AvBDs. Our observations suggest that 1,25D(3) is capable of inducing AvBD gene expression and is a potential antibiotic alternative through augmentation of host innate immunity as well as disease control in chickens.
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spelling pubmed-48529252016-05-13 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens Zhang, Long Lu, Lu Li, Siming Zhang, Guolong Ouyang, Linghua Robinson, Kelsy Tang, Yanqiang Zhu, Qing Li, Diyan Hu, Yaodong Liu, Yiping PLoS One Research Article Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D(3) (1,25D(3)) is well known as a powerful HDP inducer in humans, but limited information about the effect of 1,25D(3) on HDPs in poultry is available. Here, we sought to examine whether 1,25D(3) could stimulate avian β-defensin (AvBD) expression in chickens. We used chicken embryo intestinal epithelial cells (CEIEPCs) and peripheral blood mononuclear cells (PBMCs) to study the effect of 1,25D(3) on the expression of AvBDs. We observed that 1,25D(3) is able to up-regulate the expression of several AvBDs in CEIEPCs and PBMCs, whereas it increased the amounts of AvBD4 mRNA in CEIEPCs only in the presence of lipopolysaccharide (LPS). On the other hand, LPS treatment not only inhibited the expression of CYP24A1 but also altered the expression pattern of VDR in CEIEPCs. Furthermore, AvBDs were not directly regulated by 1,25D(3), as cycloheximide completely blocked 1,25D(3)-induced expression of AvBDs. Our observations suggest that 1,25D(3) is capable of inducing AvBD gene expression and is a potential antibiotic alternative through augmentation of host innate immunity as well as disease control in chickens. Public Library of Science 2016-05-02 /pmc/articles/PMC4852925/ /pubmed/27135828 http://dx.doi.org/10.1371/journal.pone.0154546 Text en © 2016 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Long
Lu, Lu
Li, Siming
Zhang, Guolong
Ouyang, Linghua
Robinson, Kelsy
Tang, Yanqiang
Zhu, Qing
Li, Diyan
Hu, Yaodong
Liu, Yiping
1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title_full 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title_fullStr 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title_full_unstemmed 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title_short 1,25-Dihydroxyvitamin-D(3) Induces Avian β-Defensin Gene Expression in Chickens
title_sort 1,25-dihydroxyvitamin-d(3) induces avian β-defensin gene expression in chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852925/
https://www.ncbi.nlm.nih.gov/pubmed/27135828
http://dx.doi.org/10.1371/journal.pone.0154546
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