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3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus

Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a comp...

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Autores principales: Kankam, Frederick, Long, Hai-Tao, He, Jing, Zhang, Chun-hong, Zhang, Hui-Xiu, Pu, Lumei, Qiu, Huizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853098/
https://www.ncbi.nlm.nih.gov/pubmed/27147928
http://dx.doi.org/10.5423/PPJ.OA.08.2015.0159
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author Kankam, Frederick
Long, Hai-Tao
He, Jing
Zhang, Chun-hong
Zhang, Hui-Xiu
Pu, Lumei
Qiu, Huizhen
author_facet Kankam, Frederick
Long, Hai-Tao
He, Jing
Zhang, Chun-hong
Zhang, Hui-Xiu
Pu, Lumei
Qiu, Huizhen
author_sort Kankam, Frederick
collection PubMed
description Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and 30°C. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0–8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue.
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spelling pubmed-48530982016-05-04 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus Kankam, Frederick Long, Hai-Tao He, Jing Zhang, Chun-hong Zhang, Hui-Xiu Pu, Lumei Qiu, Huizhen Plant Pathol J Research Article Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and 30°C. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0–8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue. Korean Society of Plant Pathology 2016-04 2016-04-01 /pmc/articles/PMC4853098/ /pubmed/27147928 http://dx.doi.org/10.5423/PPJ.OA.08.2015.0159 Text en © The Korean Society of Plant Pathology This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kankam, Frederick
Long, Hai-Tao
He, Jing
Zhang, Chun-hong
Zhang, Hui-Xiu
Pu, Lumei
Qiu, Huizhen
3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title_full 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title_fullStr 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title_full_unstemmed 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title_short 3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
title_sort 3-methylthiopropionic acid of rhizoctonia solani ag-3 and its role in the pathogenicity of the fungus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853098/
https://www.ncbi.nlm.nih.gov/pubmed/27147928
http://dx.doi.org/10.5423/PPJ.OA.08.2015.0159
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