Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants

OBJECTIVES: To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants. RESULTS: A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established thro...

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Detalles Bibliográficos
Autores principales: Elshikh, Mohamed, Ahmed, Syed, Funston, Scott, Dunlop, Paul, McGaw, Mark, Marchant, Roger, Banat, Ibrahim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853446/
https://www.ncbi.nlm.nih.gov/pubmed/26969604
http://dx.doi.org/10.1007/s10529-016-2079-2
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author Elshikh, Mohamed
Ahmed, Syed
Funston, Scott
Dunlop, Paul
McGaw, Mark
Marchant, Roger
Banat, Ibrahim M.
author_facet Elshikh, Mohamed
Ahmed, Syed
Funston, Scott
Dunlop, Paul
McGaw, Mark
Marchant, Roger
Banat, Ibrahim M.
author_sort Elshikh, Mohamed
collection PubMed
description OBJECTIVES: To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants. RESULTS: A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains. CONCLUSION: This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.
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spelling pubmed-48534462016-05-24 Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants Elshikh, Mohamed Ahmed, Syed Funston, Scott Dunlop, Paul McGaw, Mark Marchant, Roger Banat, Ibrahim M. Biotechnol Lett Original Research Paper OBJECTIVES: To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants. RESULTS: A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains. CONCLUSION: This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts. Springer Netherlands 2016-03-11 2016 /pmc/articles/PMC4853446/ /pubmed/26969604 http://dx.doi.org/10.1007/s10529-016-2079-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Research Paper
Elshikh, Mohamed
Ahmed, Syed
Funston, Scott
Dunlop, Paul
McGaw, Mark
Marchant, Roger
Banat, Ibrahim M.
Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title_full Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title_fullStr Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title_full_unstemmed Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title_short Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
title_sort resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853446/
https://www.ncbi.nlm.nih.gov/pubmed/26969604
http://dx.doi.org/10.1007/s10529-016-2079-2
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