High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica

We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The...

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Autores principales: Geng, Shizhong, Tian, Qin, An, Shuming, Pan, Zhiming, Chen, Xiang, Jiao, Xinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853454/
https://www.ncbi.nlm.nih.gov/pubmed/26883127
http://dx.doi.org/10.1007/s00284-016-1002-3
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author Geng, Shizhong
Tian, Qin
An, Shuming
Pan, Zhiming
Chen, Xiang
Jiao, Xinan
author_facet Geng, Shizhong
Tian, Qin
An, Shuming
Pan, Zhiming
Chen, Xiang
Jiao, Xinan
author_sort Geng, Shizhong
collection PubMed
description We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The high efficiency of this genetic engineering strategy permits the study of gene function by gene knockin, site-directed mutagenesis, and gene knockout to construct live attenuated vaccines.
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spelling pubmed-48534542016-05-24 High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica Geng, Shizhong Tian, Qin An, Shuming Pan, Zhiming Chen, Xiang Jiao, Xinan Curr Microbiol Article We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The high efficiency of this genetic engineering strategy permits the study of gene function by gene knockin, site-directed mutagenesis, and gene knockout to construct live attenuated vaccines. Springer US 2016-02-16 2016 /pmc/articles/PMC4853454/ /pubmed/26883127 http://dx.doi.org/10.1007/s00284-016-1002-3 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Geng, Shizhong
Tian, Qin
An, Shuming
Pan, Zhiming
Chen, Xiang
Jiao, Xinan
High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title_full High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title_fullStr High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title_full_unstemmed High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title_short High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica
title_sort high-efficiency, two-step scarless–markerless genome genetic modification in salmonella enterica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853454/
https://www.ncbi.nlm.nih.gov/pubmed/26883127
http://dx.doi.org/10.1007/s00284-016-1002-3
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