High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica

We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Geng, Shizhong, Tian, Qin, An, Shuming, Pan, Zhiming, Chen, Xiang, Jiao, Xinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853454/
https://www.ncbi.nlm.nih.gov/pubmed/26883127
http://dx.doi.org/10.1007/s00284-016-1002-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853454/
https://www.ncbi.nlm.nih.gov/pubmed/26883127
http://dx.doi.org/10.1007/s00284-016-1002-3

Ejemplares similares