Cargando…

High-Efficiency, Two-Step Scarless–Markerless Genome Genetic Modification in Salmonella enterica

We present a two-step method for scarless–markerless genome genetic modification in Salmonella enterica based on the improved suicide plasmid pGMB152. The whole LacZYA gene can provide a lacZ-based blue/white screening strategy for fast selection of double-crossover mutants by allelic exchange. The...

Descripción completa

Detalles Bibliográficos
Autores principales:	Geng, Shizhong, Tian, Qin, An, Shuming, Pan, Zhiming, Chen, Xiang, Jiao, Xinan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer US 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853454/ https://www.ncbi.nlm.nih.gov/pubmed/26883127 http://dx.doi.org/10.1007/s00284-016-1002-3

Ejemplares similares

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome
por: Blank, Kathrin, et al.
Publicado: (2011)

Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate
por: Yin, Junlei, et al.
Publicado: (2015)

A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica
por: Kim, Juhan, et al.
Publicado: (2014)

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay
por: Xiong, Dan, et al.
Publicado: (2018)

One-Step PCR Detection of Salmonella Pullorum/Gallinarum Using a Novel Target: The Flagellar Biosynthesis Gene flhB
por: Xiong, Dan, et al.
Publicado: (2016)

Signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of Salmonella enterica serovar Enteritidis
por: Jiao, Yang, et al.
Publicado: (2017)

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study
por: Wang, Fuzhong, et al.
Publicado: (2022)

An Efficient Multiplex PCR-Based Assay as a Novel Tool for Accurate Inter-Serovar Discrimination of Salmonella Enteritidis, S. Pullorum/Gallinarum and S. Dublin
por: Xiong, Dan, et al.
Publicado: (2017)

High genetic similarity of Salmonella Enteritidis as a predominant serovar by an independent survey in 3 large-scale chicken farms in China
por: Zhang, Jian, et al.
Publicado: (2021)

A new multiplex PCR for the accurate identification and differentiation of Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum
por: Xiong, Dan, et al.
Publicado: (2022)

Controversy Surrounding the Function of SpiC Protein in Salmonella: An Overview
por: Wang, Yaonan, et al.
Publicado: (2019)

A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB
por: Xiong, Dan, et al.
Publicado: (2015)

Safety, Protective Immunity, and DIVA Capability of a Rough Mutant Salmonella Pullorum Vaccine Candidate in Broilers
por: Guo, Rongxian, et al.
Publicado: (2017)

NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens
por: Stukenberg, Daniel, et al.
Publicado: (2022)

A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken
por: Xia, Zemiao, et al.
Publicado: (2020)

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430
por: Song, Li, et al.
Publicado: (2023)

Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome
por: Cox, Mandy M, et al.
Publicado: (2007)

Pig as a reservoir of CRISPR type TST4 Salmonella enterica serovar Typhimurium monophasic variant during 2009–2017 in China
por: Xie, Xiaolei, et al.
Publicado: (2019)

Epidemic patterns of antimicrobial resistance of Salmonella enterica serovar Gallinarum biovar Pullorum isolates in China during the past half-century
por: Sun, Fan, et al.
Publicado: (2020)

Safety and protective efficacy of Salmonella Pullorum spiC and rfaH deletion rough mutant as a live attenuated DIVA vaccine candidate
por: Kang, Xilong, et al.
Publicado: (2021)

Loss and Gain in the Evolution of the Salmonella enterica Serovar Gallinarum Biovar Pullorum Genome
por: Hu, Yachen, et al.
Publicado: (2019)

Is the Evolution of Salmonella enterica subsp. enterica Linked to Restriction-Modification Systems?
por: Roer, Louise, et al.
Publicado: (2016)

Identification and Immune Functional Characterization of Pigeon TLR7
por: Xiong, Dan, et al.
Publicado: (2015)

Nifuratel reduces Salmonella survival in macrophages by extracellular and intracellular antibacterial activity
por: Xie, Tian, et al.
Publicado: (2023)

Activation of TGF-β-activated kinase 1 (TAK1) restricts Salmonella Typhimurium growth by inducing AMPK activation and autophagy
por: Liu, Wei, et al.
Publicado: (2018)

Construction of gene modification system with highly efficient and markerless for Monascus ruber M7
por: Xu, Na, et al.
Publicado: (2022)

Roles of the spiA gene from Salmonella enteritidis in biofilm formation and virulence
por: Dong, Hongyan, et al.
Publicado: (2011)

Revisiting Persistent Salmonella Infection and the Carrier State: What Do We Know?
por: Foster, Neil, et al.
Publicado: (2021)

Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica
por: de Moraes, Marcos H, et al.
Publicado: (2015)

An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
por: Geng, Shi-Zhong, et al.
Publicado: (2009)

An efficient, scarless, selection-free technology for phage engineering
por: Goren, Moran G., et al.
Publicado: (2023)

Recombinant Salmonella expressing SspH2-EscI fusion protein limits its colonization in mice
por: Hu, Maozhi, et al.
Publicado: (2017)

Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
por: He, Wei, et al.
Publicado: (2015)

Rates of Mutations and Transcript Errors in the Foodborne Pathogen Salmonella enterica subsp. enterica
por: Pan, Jiao, et al.
Publicado: (2022)

“Scarless” Inguinal Herniorrhaphy
por: Abdel-Aziz, Hiba, et al.
Publicado: (2017)

Preclinical evaluation of OMVs as potential vaccine candidates against Salmonella enterica serovar Enteritidis infection
por: Jiang, Xi, et al.
Publicado: (2022)

Is the future scarless? – Fibroblasts as targets for scarless wound healing: a narrative review
por: Parry, Dylan, et al.
Publicado: (2022)

Complete Genome Sequence of Salmonella enterica Serovar Choleraesuis Vaccine Strain C500 Attenuated by Chemical Mutation
por: Li, Qiuchun, et al.
Publicado: (2014)

Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe
por: Landgraf, Dirk, et al.
Publicado: (2016)

Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and Salmonella enterica subsp enterica serovar Gallinarum
por: Ribeiro, Simone Alves Mendes, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_vjb68ifal72pn1lmirfqa8hqif