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Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells
Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate the genotoxic potential of BA and its effect on the genotoxicity induced by different mutagens in V79 cells using the cytokinesis-block micronucleus...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853943/ https://www.ncbi.nlm.nih.gov/pubmed/27195016 http://dx.doi.org/10.1155/2016/8942730 |
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author | Acésio, Nathália Oliveira de Oliveira, Pollyanna Francielli Mastrocola, Daiane Fernanda Pereira Lima, Ildercílio Mota de Souza Munari, Carla Carolina Sato, Vânia Luiza Ferreira Lucatti Souza, Andressa Aparecida Silva Flauzino, Lúzio Gabriel Bocalon Cunha, Wilson Roberto Tavares, Denise Crispim |
author_facet | Acésio, Nathália Oliveira de Oliveira, Pollyanna Francielli Mastrocola, Daiane Fernanda Pereira Lima, Ildercílio Mota de Souza Munari, Carla Carolina Sato, Vânia Luiza Ferreira Lucatti Souza, Andressa Aparecida Silva Flauzino, Lúzio Gabriel Bocalon Cunha, Wilson Roberto Tavares, Denise Crispim |
author_sort | Acésio, Nathália Oliveira |
collection | PubMed |
description | Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate the genotoxic potential of BA and its effect on the genotoxicity induced by different mutagens in V79 cells using the cytokinesis-block micronucleus assay. Different BA concentrations were combined with methyl methanesulfonate (MMS), doxorubicin (DXR), camptothecin (CPT), and etoposide (VP-16). The frequencies of micronuclei in cultures treated with different BA concentrations did not differ from those of the negative control. Treatment with BA and MMS resulted in lower micronucleus frequencies than those observed for cultures treated with MMS alone. On the other hand, a significant increase in micronucleus frequencies was observed in cultures treated with BA combined with DXR or VP-16 when compared to these mutagens alone. The results showed no effect of BA on CPT-induced genotoxicity. Therefore, BA was not genotoxic under the present experimental conditions and exerted a different influence on the genotoxicity induced by different mutagens. The modulatory effect of BA depends on the type of mutagen and concentrations used. |
format | Online Article Text |
id | pubmed-4853943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48539432016-05-18 Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells Acésio, Nathália Oliveira de Oliveira, Pollyanna Francielli Mastrocola, Daiane Fernanda Pereira Lima, Ildercílio Mota de Souza Munari, Carla Carolina Sato, Vânia Luiza Ferreira Lucatti Souza, Andressa Aparecida Silva Flauzino, Lúzio Gabriel Bocalon Cunha, Wilson Roberto Tavares, Denise Crispim Evid Based Complement Alternat Med Research Article Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate the genotoxic potential of BA and its effect on the genotoxicity induced by different mutagens in V79 cells using the cytokinesis-block micronucleus assay. Different BA concentrations were combined with methyl methanesulfonate (MMS), doxorubicin (DXR), camptothecin (CPT), and etoposide (VP-16). The frequencies of micronuclei in cultures treated with different BA concentrations did not differ from those of the negative control. Treatment with BA and MMS resulted in lower micronucleus frequencies than those observed for cultures treated with MMS alone. On the other hand, a significant increase in micronucleus frequencies was observed in cultures treated with BA combined with DXR or VP-16 when compared to these mutagens alone. The results showed no effect of BA on CPT-induced genotoxicity. Therefore, BA was not genotoxic under the present experimental conditions and exerted a different influence on the genotoxicity induced by different mutagens. The modulatory effect of BA depends on the type of mutagen and concentrations used. Hindawi Publishing Corporation 2016 2016-04-19 /pmc/articles/PMC4853943/ /pubmed/27195016 http://dx.doi.org/10.1155/2016/8942730 Text en Copyright © 2016 Nathália Oliveira Acésio et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Acésio, Nathália Oliveira de Oliveira, Pollyanna Francielli Mastrocola, Daiane Fernanda Pereira Lima, Ildercílio Mota de Souza Munari, Carla Carolina Sato, Vânia Luiza Ferreira Lucatti Souza, Andressa Aparecida Silva Flauzino, Lúzio Gabriel Bocalon Cunha, Wilson Roberto Tavares, Denise Crispim Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title | Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title_full | Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title_fullStr | Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title_full_unstemmed | Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title_short | Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells |
title_sort | modulatory effect of betulinic acid on the genotoxicity induced by different mutagens in v79 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853943/ https://www.ncbi.nlm.nih.gov/pubmed/27195016 http://dx.doi.org/10.1155/2016/8942730 |
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