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Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells

Paeoniflorin (PF) is a monoterpene glycoside extracted from the root of Paeonia lactiflora Pall. Previous studies have demonstrated that PF inhibits the growth, invasion, and metastasis of tumors in vivo and in vitro. However, the effect of PF on hypoxia-induced epithelial–mesenchymal transition (EM...

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Autores principales: Zhou, Zhenyu, Wang, Shunchang, Song, Caijuan, Hu, Zhuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854234/
https://www.ncbi.nlm.nih.gov/pubmed/27175085
http://dx.doi.org/10.2147/OTT.S102422
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author Zhou, Zhenyu
Wang, Shunchang
Song, Caijuan
Hu, Zhuang
author_facet Zhou, Zhenyu
Wang, Shunchang
Song, Caijuan
Hu, Zhuang
author_sort Zhou, Zhenyu
collection PubMed
description Paeoniflorin (PF) is a monoterpene glycoside extracted from the root of Paeonia lactiflora Pall. Previous studies have demonstrated that PF inhibits the growth, invasion, and metastasis of tumors in vivo and in vitro. However, the effect of PF on hypoxia-induced epithelial–mesenchymal transition (EMT) in breast cancer cells remains unknown. Therefore, the objective of this study was to investigate the effect of PF on hypoxia-induced EMT in breast cancer cells, as well as characterize the underlying mechanism. The results presented in this study demonstrate that PF blocks the migration and invasion of breast cancer cells by repressing EMT under hypoxic conditions. PF also significantly attenuated the hypoxia-induced increase in HIF-1α level. Furthermore, PF prevented hypoxia-induced expression of phosphorylated PI3K and Akt in MDA-MB-231 cells. In conclusion, PF prevented hypoxia-induced EMT in breast cancer cells by inhibiting HIF-1α expression via modulation of PI3K/Akt signaling pathway. This finding provides evidence that PF can serve as a therapeutic agent for the treatment of breast cancer.
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spelling pubmed-48542342016-05-12 Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells Zhou, Zhenyu Wang, Shunchang Song, Caijuan Hu, Zhuang Onco Targets Ther Original Research Paeoniflorin (PF) is a monoterpene glycoside extracted from the root of Paeonia lactiflora Pall. Previous studies have demonstrated that PF inhibits the growth, invasion, and metastasis of tumors in vivo and in vitro. However, the effect of PF on hypoxia-induced epithelial–mesenchymal transition (EMT) in breast cancer cells remains unknown. Therefore, the objective of this study was to investigate the effect of PF on hypoxia-induced EMT in breast cancer cells, as well as characterize the underlying mechanism. The results presented in this study demonstrate that PF blocks the migration and invasion of breast cancer cells by repressing EMT under hypoxic conditions. PF also significantly attenuated the hypoxia-induced increase in HIF-1α level. Furthermore, PF prevented hypoxia-induced expression of phosphorylated PI3K and Akt in MDA-MB-231 cells. In conclusion, PF prevented hypoxia-induced EMT in breast cancer cells by inhibiting HIF-1α expression via modulation of PI3K/Akt signaling pathway. This finding provides evidence that PF can serve as a therapeutic agent for the treatment of breast cancer. Dove Medical Press 2016-04-27 /pmc/articles/PMC4854234/ /pubmed/27175085 http://dx.doi.org/10.2147/OTT.S102422 Text en © 2016 Zhou et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhou, Zhenyu
Wang, Shunchang
Song, Caijuan
Hu, Zhuang
Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title_full Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title_fullStr Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title_full_unstemmed Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title_short Paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
title_sort paeoniflorin prevents hypoxia-induced epithelial–mesenchymal transition in human breast cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854234/
https://www.ncbi.nlm.nih.gov/pubmed/27175085
http://dx.doi.org/10.2147/OTT.S102422
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