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Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization
The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854439/ https://www.ncbi.nlm.nih.gov/pubmed/27138472 http://dx.doi.org/10.1371/journal.pone.0154643 |
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author | Zhong, Yu Anderl, Florian Kruse, Tobias Schindele, Franziska Jagusztyn-Krynicka, Elżbieta Katarzyna Fischer, Wolfgang Gerhard, Markus Mejías-Luque, Raquel |
author_facet | Zhong, Yu Anderl, Florian Kruse, Tobias Schindele, Franziska Jagusztyn-Krynicka, Elżbieta Katarzyna Fischer, Wolfgang Gerhard, Markus Mejías-Luque, Raquel |
author_sort | Zhong, Yu |
collection | PubMed |
description | The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many H. pylori virulence factors are stabilized by the formation of disulfide bonds. By employing H. pylori HP0231 deficient strains we analyzed the effect of lack of this bacterial protein on the functionality of virulence factors containing putative disulfide bonds. The lack of H. pylori HP0231 impaired CagA translocation into gastric epithelial cells and reduced VacA-induced cellular vacuolation. Moreover, H. pylori HP0231 deficient bacteria were not able to colonize the gastric mucosa of mice, probably due to compromised motility. Together, our data demonstrate an essential function for H. pylori HP0231 in gastric colonization and proper function of bacterial virulence factors related to gastric pathology. |
format | Online Article Text |
id | pubmed-4854439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48544392016-05-07 Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization Zhong, Yu Anderl, Florian Kruse, Tobias Schindele, Franziska Jagusztyn-Krynicka, Elżbieta Katarzyna Fischer, Wolfgang Gerhard, Markus Mejías-Luque, Raquel PLoS One Research Article The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many H. pylori virulence factors are stabilized by the formation of disulfide bonds. By employing H. pylori HP0231 deficient strains we analyzed the effect of lack of this bacterial protein on the functionality of virulence factors containing putative disulfide bonds. The lack of H. pylori HP0231 impaired CagA translocation into gastric epithelial cells and reduced VacA-induced cellular vacuolation. Moreover, H. pylori HP0231 deficient bacteria were not able to colonize the gastric mucosa of mice, probably due to compromised motility. Together, our data demonstrate an essential function for H. pylori HP0231 in gastric colonization and proper function of bacterial virulence factors related to gastric pathology. Public Library of Science 2016-05-03 /pmc/articles/PMC4854439/ /pubmed/27138472 http://dx.doi.org/10.1371/journal.pone.0154643 Text en © 2016 Zhong et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhong, Yu Anderl, Florian Kruse, Tobias Schindele, Franziska Jagusztyn-Krynicka, Elżbieta Katarzyna Fischer, Wolfgang Gerhard, Markus Mejías-Luque, Raquel Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title | Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title_full | Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title_fullStr | Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title_full_unstemmed | Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title_short | Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization |
title_sort | helicobacter pylori hp0231 influences bacterial virulence and is essential for gastric colonization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854439/ https://www.ncbi.nlm.nih.gov/pubmed/27138472 http://dx.doi.org/10.1371/journal.pone.0154643 |
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