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Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures
A variety of mutants having different colony characteristics, morphology and soluble pigmentation were generated from Fusarium fujikuroi by exposure to UV radiation. Mutants were selected that formed dry, compact, small colonies with reddish-violet pigment on regeneration agar plates. The production...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854849/ https://www.ncbi.nlm.nih.gov/pubmed/27142994 http://dx.doi.org/10.1186/s13568-016-0205-0 |
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author | Lale, G. J. Gadre, R. V. |
author_facet | Lale, G. J. Gadre, R. V. |
author_sort | Lale, G. J. |
collection | PubMed |
description | A variety of mutants having different colony characteristics, morphology and soluble pigmentation were generated from Fusarium fujikuroi by exposure to UV radiation. Mutants were selected that formed dry, compact, small colonies with reddish-violet pigment on regeneration agar plates. The production of bikaverin by Mut-4 was examined in shake flasks in media with different nitrogen and carbon sources. The optimal C: N ratio for the maximal bikaverin production by Mut-4 was 150:1. It produced still higher bikaverin (6.3 g l(−1)) in a medium containing defatted cottonseed meal as nitrogen source, in combination with glucose. Bikaverin produced was extracted, purified and characterized by UV-visible and NMR spectroscopy. Bikaverin production in the present investigation was substantially higher than that reported by earlier investigators in submerged and solid-state fermentations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0205-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4854849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-48548492016-06-14 Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures Lale, G. J. Gadre, R. V. AMB Express Original Article A variety of mutants having different colony characteristics, morphology and soluble pigmentation were generated from Fusarium fujikuroi by exposure to UV radiation. Mutants were selected that formed dry, compact, small colonies with reddish-violet pigment on regeneration agar plates. The production of bikaverin by Mut-4 was examined in shake flasks in media with different nitrogen and carbon sources. The optimal C: N ratio for the maximal bikaverin production by Mut-4 was 150:1. It produced still higher bikaverin (6.3 g l(−1)) in a medium containing defatted cottonseed meal as nitrogen source, in combination with glucose. Bikaverin produced was extracted, purified and characterized by UV-visible and NMR spectroscopy. Bikaverin production in the present investigation was substantially higher than that reported by earlier investigators in submerged and solid-state fermentations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0205-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-05-03 /pmc/articles/PMC4854849/ /pubmed/27142994 http://dx.doi.org/10.1186/s13568-016-0205-0 Text en © Lale and Gadre. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Lale, G. J. Gadre, R. V. Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title | Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title_full | Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title_fullStr | Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title_full_unstemmed | Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title_short | Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures |
title_sort | production of bikaverin by a fusarium fujikuroi mutant in submerged cultures |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854849/ https://www.ncbi.nlm.nih.gov/pubmed/27142994 http://dx.doi.org/10.1186/s13568-016-0205-0 |
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