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Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae

Streptococcus pneumoniae is a Gram-positive microorganism that is the cause of bacterial pneumonia, sinusitis and otitis media. This human pathogen also can cause invasive diseases such as meningitis, bacteremia and septicemia. Hemoglobin (Hb) and haem can support the growth and viability of S. pneu...

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Autores principales: Romero-Espejel, María E., Rodríguez, Mario A., Chávez-Munguía, Bibiana, Ríos-Castro, Emmanuel, Olivares-Trejo, José de Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854876/
https://www.ncbi.nlm.nih.gov/pubmed/27200302
http://dx.doi.org/10.3389/fcimb.2016.00047
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author Romero-Espejel, María E.
Rodríguez, Mario A.
Chávez-Munguía, Bibiana
Ríos-Castro, Emmanuel
Olivares-Trejo, José de Jesús
author_facet Romero-Espejel, María E.
Rodríguez, Mario A.
Chávez-Munguía, Bibiana
Ríos-Castro, Emmanuel
Olivares-Trejo, José de Jesús
author_sort Romero-Espejel, María E.
collection PubMed
description Streptococcus pneumoniae is a Gram-positive microorganism that is the cause of bacterial pneumonia, sinusitis and otitis media. This human pathogen also can cause invasive diseases such as meningitis, bacteremia and septicemia. Hemoglobin (Hb) and haem can support the growth and viability of S. pneumoniae as sole iron sources. Unfortunately, the acquisition mechanism of Hb and haem in this bacterium has been poorly studied. Previously we identified two proteins of 37 and 22 kDa as putative Hb- and haem-binding proteins (Spbhp-37 and Spbhp-22, respectively). The sequence of Spbhp-37 protein was database annotated as lipoprotein without any function or localization. Here it was immunolocalized in the surface cell by transmission electron microscopy using specific antibodies produced against the recombinant protein. The expression of Spbhp-37 was increased when bacteria were grown in media culture supplied with Hb. In addition, the affinity of Sphbp-37 for Hb was determined. Thus, in this work we are presenting new findings that attempt to explain the mechanism involved in iron acquisition of this pathogen. In the future these results could help to develop new therapy targets in order to avoid the secondary effects caused by the traditional therapies.
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spelling pubmed-48548762016-05-19 Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae Romero-Espejel, María E. Rodríguez, Mario A. Chávez-Munguía, Bibiana Ríos-Castro, Emmanuel Olivares-Trejo, José de Jesús Front Cell Infect Microbiol Microbiology Streptococcus pneumoniae is a Gram-positive microorganism that is the cause of bacterial pneumonia, sinusitis and otitis media. This human pathogen also can cause invasive diseases such as meningitis, bacteremia and septicemia. Hemoglobin (Hb) and haem can support the growth and viability of S. pneumoniae as sole iron sources. Unfortunately, the acquisition mechanism of Hb and haem in this bacterium has been poorly studied. Previously we identified two proteins of 37 and 22 kDa as putative Hb- and haem-binding proteins (Spbhp-37 and Spbhp-22, respectively). The sequence of Spbhp-37 protein was database annotated as lipoprotein without any function or localization. Here it was immunolocalized in the surface cell by transmission electron microscopy using specific antibodies produced against the recombinant protein. The expression of Spbhp-37 was increased when bacteria were grown in media culture supplied with Hb. In addition, the affinity of Sphbp-37 for Hb was determined. Thus, in this work we are presenting new findings that attempt to explain the mechanism involved in iron acquisition of this pathogen. In the future these results could help to develop new therapy targets in order to avoid the secondary effects caused by the traditional therapies. Frontiers Media S.A. 2016-05-04 /pmc/articles/PMC4854876/ /pubmed/27200302 http://dx.doi.org/10.3389/fcimb.2016.00047 Text en Copyright © 2016 Romero-Espejel, Rodríguez, Chávez-Munguía, Ríos-Castro and Olivares-Trejo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Romero-Espejel, María E.
Rodríguez, Mario A.
Chávez-Munguía, Bibiana
Ríos-Castro, Emmanuel
Olivares-Trejo, José de Jesús
Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title_full Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title_fullStr Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title_full_unstemmed Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title_short Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae
title_sort characterization of spbhp-37, a hemoglobin-binding protein of streptococcus pneumoniae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854876/
https://www.ncbi.nlm.nih.gov/pubmed/27200302
http://dx.doi.org/10.3389/fcimb.2016.00047
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