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A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity
BACKGROUND: Bacterial surface display is an attractive technique for the production of cell-anchored, functional proteins and engineering of whole-cell catalysts. Although various outer membrane proteins have been used for surface display, an easy and versatile high-throughput-compatible assay for e...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855350/ https://www.ncbi.nlm.nih.gov/pubmed/27142225 http://dx.doi.org/10.1186/s12934-016-0474-y |