Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells

A multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (SF) of irradiated cells. The estimation of SF based on the cell-growth curve information is the major advantage of this assay. In this study, the utility of multiple-MTS assay for...

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Autores principales: Arab-Bafrani, Zahra, Shahbazi-Gahrouei, Daryoush, Abbasian, Mahdi, Fesharaki, Mehrafarin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855884/
https://www.ncbi.nlm.nih.gov/pubmed/27186539
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author Arab-Bafrani, Zahra
Shahbazi-Gahrouei, Daryoush
Abbasian, Mahdi
Fesharaki, Mehrafarin
author_facet Arab-Bafrani, Zahra
Shahbazi-Gahrouei, Daryoush
Abbasian, Mahdi
Fesharaki, Mehrafarin
author_sort Arab-Bafrani, Zahra
collection PubMed
description A multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (SF) of irradiated cells. The estimation of SF based on the cell-growth curve information is the major advantage of this assay. In this study, the utility of multiple-MTS assay for the SF estimation of irradiated HT-29 colon cancer cells, which were plated before irradiation, was evaluated. The SF of HT-29 colon cancer cells under irradiation with 9 MV photon was estimated using multiple-MTS assay and colony assay. Finally, the correlation between two assays was evaluated. Results showed that there are no significant differences between the SF obtained by two assays at different radiation doses (P > 0.05), and the survival curves have quite similar trends. In conclusion, multiple MTS-assay can be a reliable method to determine the SF of irradiated colon cancer cells that plated before irradiation.
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spelling pubmed-48558842016-05-16 Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells Arab-Bafrani, Zahra Shahbazi-Gahrouei, Daryoush Abbasian, Mahdi Fesharaki, Mehrafarin J Med Signals Sens Original Article A multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (SF) of irradiated cells. The estimation of SF based on the cell-growth curve information is the major advantage of this assay. In this study, the utility of multiple-MTS assay for the SF estimation of irradiated HT-29 colon cancer cells, which were plated before irradiation, was evaluated. The SF of HT-29 colon cancer cells under irradiation with 9 MV photon was estimated using multiple-MTS assay and colony assay. Finally, the correlation between two assays was evaluated. Results showed that there are no significant differences between the SF obtained by two assays at different radiation doses (P > 0.05), and the survival curves have quite similar trends. In conclusion, multiple MTS-assay can be a reliable method to determine the SF of irradiated colon cancer cells that plated before irradiation. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4855884/ /pubmed/27186539 Text en Copyright: © 2016 Journal of Medical Signals & Sensors http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Arab-Bafrani, Zahra
Shahbazi-Gahrouei, Daryoush
Abbasian, Mahdi
Fesharaki, Mehrafarin
Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title_full Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title_fullStr Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title_full_unstemmed Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title_short Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells
title_sort multiple mts assay as the alternative method to determine survival fraction of the irradiated ht-29 colon cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4855884/
https://www.ncbi.nlm.nih.gov/pubmed/27186539
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